METHODS: Sixteen mice were divided into four groups, and EVE was performed at different time points before or both before and after fat grafting. Fat tissue from a human donor was injected into the mice following EVE. Visual assessment, micro-computed tomography analysis, and histopathological evaluation were used to assess fat retention.
RESULTS: After 10 weeks, the group that underwent EVE 5 days before fat grafting demonstrated a significantly higher preserved fat volume, as determined by micro-computed tomography (p<0.05). Moreover, the group that received additional EVE after fat grafting exhibited a higher retention rate compared to the groups receiving EVE only before grafting (p<0.05). Histopathological analysis indicated that swelling, edema, and inflammation were more pronounced in the group with EVE immediately before grafting, while angiogenesis and lipogenesis were more active in the group with additional EVE after grafting.
CONCLUSIONS: EVE is a safe and effective approach for improving the rate of fat graft retentions. Furthermore, the timing of external tissue expansion plays a crucial role in fat retention. Based on our animal study, performing EVE immediately before and after fat grafting may be an effective strategy for enhancing the rate of fat graft retentions.
方法:将16只小鼠分为四组,和EVE在脂肪移植之前或之前和之后的不同时间点进行。EVE后将来自人供体的脂肪组织注射到小鼠中。视觉评估,显微计算机断层扫描分析,和组织病理学评估用于评估脂肪保留。
结果:10周后,在脂肪移植前5天接受EVE的组显示出明显更高的保留脂肪量,通过显微计算机断层扫描确定(p<0.05)。此外,与仅在移植前接受EVE的组相比,在脂肪移植后接受额外EVE的组表现出更高的保留率(p<0.05).组织病理学分析显示肿胀,水肿,移植前EVE组炎症更明显,而血管生成和脂肪生成在移植后增加EVE的组中更活跃。
结论:EVE是一种安全有效的提高脂肪移植物保留率的方法。此外,外部组织扩张的时机在脂肪保留中起着至关重要的作用。根据我们的动物研究,在脂肪移植前后立即进行EVE可能是提高脂肪移植保留率的有效策略。