为了研究肠道消化酶的活性,肝脏抗氧化酶,免疫酶,在Biofloc模型下,大嘴鲈鱼(Micropterussalmoides)中的糖代谢酶,在300升玻璃罐中进行了一项实验。实验包括一个对照组,以基础饮食喂养,和一个biofloc组,其中添加葡萄糖以保持C/N比为15。每组有三个平行设置,每个鱼缸放养密度为20条鱼。实验持续了60天,采用零水交换水产养殖模式。结果表明,在培养期结束时,初始体重之间没有显着差异,最终重量,WGR,SGR,Biofloc组和大口鲈鱼对照组的SR(p>0.05),而biofloc组较低的FCR和较高的PER是显著的(p<0.05);肠α-淀粉酶,胰蛋白酶,Biofloc组大口鲈鱼的脂肪酶活性显着增加了37.20%,64.11%,和51.69%,分别,与对照组相比(p<0.05);肝脏超氧化物歧化酶和过氧化氢酶活性,Biofloc组大口鲈鱼的总抗氧化能力显着提高了49.26%,46.87%,98.94%(p<0.05),而丙二醛含量显着降低了19.91%(p<0.05);肝脏溶菌酶,碱性磷酸酶,Biofloc组大嘴鲈鱼的酸性磷酸酶活性显着提高了62.66%,41.22%,29.66%,分别(p<0.05);肝脏葡萄糖激酶,丙酮酸激酶,葡萄糖-6-磷酸激酶,丙酮酸激酶,葡萄糖-6-磷酸酶,糖原合成酶活性显著提高46.29%,99.33%,32.54%,和26.89%,分别为(p<0.05)。研究表明,培养大嘴鲈鱼的Biofloc模型不仅可以增强消化酶活性,抗氧化能力,和免疫反应,但也可以促进葡萄糖代谢过程和降低饲养成本。本研究为今后生产大嘴鲈鱼的健康养殖提供数据支持。为优化Biofloc技术培养模式提供了理论参考,对于促进水产养殖健康绿色发展至关重要。
To investigate the activities of intestinal
digestive enzymes, liver antioxidant enzymes, immunological enzymes, and glucometabolic enzymes in largemouth bass (Micropterus salmoides) under the biofloc model, an experiment was conducted in 300-liter glass tanks. The experiment comprised a control group, which was fed a basal diet, and a biofloc group, where glucose was added to maintain a C/N ratio of 15. Each group had three parallel setups, with a stocking density of 20 fish per tank. The experiment ran for 60 days, employing a zero-water exchange aquaculture model. The results showed that at the end of the culture period, there were no significant differences between the initial weight, final weight, WGR, SGR, and SR of the biofloc group and the control group of largemouth bass (p > 0.05), whereas the lower FCR and the higher PER in the biofloc group were significant (p < 0.05); intestinal α-amylase, trypsin, and lipase activities of largemouth bass in the biofloc group were significantly increased by 37.20%, 64.11%, and 51.69%, respectively, compared with the control group (p < 0.05); liver superoxide dismutase and catalase activities, and total antioxidant capacity of largemouth bass in the biofloc group were significantly increased by 49.26%, 46.87%, and 98.94% (p < 0.05), while the malondialdehyde content was significantly reduced by 19.91% (p < 0.05); liver lysozyme, alkaline phosphatase, and acid phosphatase activities of largemouth bass in the biofloc group were significantly increased by 62.66%, 41.22%, and 29.66%, respectively (p < 0.05); liver glucokinase, pyruvate kinase, glucose-6-phosphate kinase, pyruvate kinase, glucose-6-phosphatase, and glycogen synthase activities were significantly increased by 46.29%, 99.33%, 32.54%, and 26.89%, respectively (p < 0.05). The study showed that the biofloc model of culturing largemouth bass can not only enhance
digestive enzyme activities, antioxidant capacity, and immune response but can also promote the process of glucose metabolism and reduce feeding costs. This study provides data support for healthy culturing of largemouth bass in future production, provides a theoretical reference for optimizing the biofloc technology culture model, and is crucial for promoting the healthy and green development of aquaculture.