PDF(Pubmed)
Abstract：
Safe and anti-inflammatory plant-based natural products present an increasing focus in the treatment of chronic inflammatory diseases such as osteoarthritis or inflammatory bowel diseases. Among them, saffron, a spice derived from the stigma of Crocus sativus, could have anti-inflammatory properties and would be therefore a promising therapeutic agent for the treatment of such conditions. However, the anti-inflammatory molecular mechanisms of saffron in humans are still understudied and unclear. In this study, combining human serum metabolites and cell cultures, we evaluated the effect of circulating metabolites from the consumption of a patented saffron extract (Safr\'InsideTM) on the chondrocytes and colon epithelial cell responses to inflammatory stress. Parametric or non-parametric Analysis of Variance with post hoc tests was performed. We demonstrated that human serum containing metabolites from saffron intake attenuated IL-1β-stimulated production of PGE2 and MMP-13 in chondrocyte cells and limited the increase in ICAM-1, MCP-1, iNOS, and MMP-3 in human epithelial cells following combined IL-1β and TNF-α inflammatory stimulation. Altogether, these data provide new findings into the mechanisms underlying the beneficial effects of saffron on chondrocytes and enterocyte cells at the cellular level and in the context of chronic inflammatory disorders.
摘要：
安全和抗炎的基于植物的天然产物在慢性炎性疾病如骨关节炎或炎性肠病的治疗中日益受到关注。其中,藏红花,一种源自番红花污名的香料，可以具有抗炎特性,因此将是用于治疗此类病症的有希望的治疗剂。然而,藏红花在人类体内的抗炎分子机制尚不清楚。在这项研究中,结合人类血清代谢物和细胞培养物，我们评估了消耗专利藏红花提取物（Safr\'InsideTM）产生的循环代谢物对软骨细胞和结肠上皮细胞对炎症应激反应的影响。使用事后测试进行了参数或非参数方差分析。我们证明了藏红花摄入的含有代谢物的人血清减弱了软骨细胞中IL-1β刺激的PGE2和MMP-13的产生，并限制了ICAM-1，MCP-1，iNOS的增加。IL-1β和TNF-α联合炎症刺激后人上皮细胞中的MMP-3。总之,这些数据为藏红花在细胞水平和慢性炎症性疾病中对软骨细胞和肠上皮细胞有益作用的潜在机制提供了新的发现.
