PDF(Pubmed)
Abstract：
Roux-en-Y gastric bypasses (RYGB) are frequently accompanied by long-term gastrointestinal (GI) symptoms. Direct mechanistic insight into the causation of these symptoms is lacking, but changes in the intestinal microbiome have been proposed to play a role. With this study, we aimed to investigate whether a microbial predisposition exists before RYGB which is associated with GI symptoms during follow-up and to evaluate which microbial groups are involved.
In total, 67 RYGB patients were included. Shotgun metagenomic sequencing was performed on fecal samples obtained just before and 1 year after surgery. To assess GI symptoms, patients filled out Gastrointestinal Quality of Life Index (GIQLI) questionnaires and were divided into groups based on their total GIQLI score and change in score (postsurgery versus baseline). Extremely randomized tree predictor models were used to identify the most distinctive microbial species associated with postoperative GI symptoms.
Beta diversity differed significantly between baseline and 1-year post-surgery samples, with the post-surgery microbiome resembling a more dysbiotic profile. The most predictive species regarding total GIQLI (AUC 0.77) or delta GIQLI score (AUC 0.83) were identified. Many of these species are known butyrate producers or species known to support them and/or species with anti-inflammatory properties, including Coprococcus eutactus, Faecalibacterium prausnitzii, and Ruminococcus callidus.
Beneficial commensal gut microbiota related to a high GI score were associated to adequate intestinal fermentative capacity, suggesting these species might have protective properties against postoperative GI malfunctioning.
In total, 67 RYGB patients were included. Shotgun metagenomic sequencing was performed on fecal samples obtained just before and 1 year after surgery. To assess GI symptoms, patients filled out Gastrointestinal Quality of Life Index (GIQLI) questionnaires and were divided into groups based on their total GIQLI score and change in score (postsurgery versus baseline). Extremely randomized tree predictor models were used to identify the most distinctive microbial species associated with postoperative GI symptoms.
Beta diversity differed significantly between baseline and 1-year post-surgery samples, with the post-surgery microbiome resembling a more dysbiotic profile. The most predictive species regarding total GIQLI (AUC 0.77) or delta GIQLI score (AUC 0.83) were identified. Many of these species are known butyrate producers or species known to support them and/or species with anti-inflammatory properties, including Coprococcus eutactus, Faecalibacterium prausnitzii, and Ruminococcus callidus.
Beneficial commensal gut microbiota related to a high GI score were associated to adequate intestinal fermentative capacity, suggesting these species might have protective properties against postoperative GI malfunctioning.
摘要：
目的：Roux-en-Y胃旁路术（RYGB）常伴有长期胃肠道（GI）症状。缺乏对这些症状的因果关系的直接机械洞察，但肠道微生物组的变化已被提出发挥作用。通过这项研究,我们旨在调查RYGB之前是否存在与随访期间胃肠道症状相关的微生物易感性,并评估涉及哪些微生物群.
方法：总共，纳入67例RYGB患者。对手术前和手术后1年获得的粪便样品进行鸟枪宏基因组测序。为了评估胃肠道症状，患者填写胃肠道生活质量指数(GIQLI)问卷,并根据GIQLI总评分和评分变化(手术后与基线比较)分组.使用极随机树预测模型来鉴定与术后胃肠道症状相关的最独特的微生物种类。
结果：基线和手术后1年样本之间的β多样性差异显着，手术后的微生物组类似于更多的生态失调。鉴定了关于总GIQLI(AUC0.77)或deltaGIQLI评分(AUC0.83)的最具预测性的物种。这些物种中的许多是已知的丁酸产生者或已知支持它们的物种和/或具有抗炎特性的物种，包括肠球菌，普氏粪杆菌，和大反刍动物。
结论：与高GI评分相关的有益共生肠道菌群与足够的肠道发酵能力相关，这表明这些物种可能对术后胃肠道功能障碍具有保护作用。
方法：总共，纳入67例RYGB患者。对手术前和手术后1年获得的粪便样品进行鸟枪宏基因组测序。为了评估胃肠道症状，患者填写胃肠道生活质量指数(GIQLI)问卷,并根据GIQLI总评分和评分变化(手术后与基线比较)分组.使用极随机树预测模型来鉴定与术后胃肠道症状相关的最独特的微生物种类。
结果：基线和手术后1年样本之间的β多样性差异显着，手术后的微生物组类似于更多的生态失调。鉴定了关于总GIQLI(AUC0.77)或deltaGIQLI评分(AUC0.83)的最具预测性的物种。这些物种中的许多是已知的丁酸产生者或已知支持它们的物种和/或具有抗炎特性的物种，包括肠球菌，普氏粪杆菌，和大反刍动物。
结论：与高GI评分相关的有益共生肠道菌群与足够的肠道发酵能力相关，这表明这些物种可能对术后胃肠道功能障碍具有保护作用。
