PDF(Pubmed)
Abstract：
Artificial magnetic fields are unavoidable environment for offshore marine organisms. With the substantially increasing submarine cables, the impact of magnetic field generated by cables on marine organisms has gradually attracted people\'s attention. However, there are few studies on the effect of magnetic field on molluscs. To explore whether magnetic fields could interfere with the physiological functions of offshore molluscs, here we systematically analyzed the change of metabolism and transcriptome of Elysia leucolegnote exposed to either geomagnetic field or 1.1 T static magnetic field. The blood glucose and lipid levels, as well as the activities of antioxidant enzymes in E. leucolegnote were significantly increased upon the exposure to high static magnetic field for 10 days. Meanwhile, the activities of enzymes related to digestive performance and liver functions were decreased. Possible mechanisms were further revealed through comparative transcriptome analysis. A total of 836 differentially expressed genes were identified, 352 of which were up-regulated and 484 of which were down-regulated after exposure to the high static magnetic field. The up-regulated differential genes were mainly concentrated in lysosomal and apoptotic pathways, and down-regulated differential genes were mainly involved in digestive and immune systems including phagocytosis. This pattern was further confirmed by RT-qPCR analysis. In conclusion, prolonged exposure to a 1.1 T static magnetic field increased oxidative stress and blood glucose and lipid levels, and decreased immunity and physiological conditions in E. leucolegnote. The data we presented here provides a comprehensive view of metabolism change and gene expression pattern of E. leucolegnote exposed to static magnetic field. It may expand our knowledge on the magnetic field effects on offshore mollusc at molecular level, and contribute to clarification of the interaction between marine animals and artificial magnetic fields, which is certainly ecologically important.
摘要：
人工磁场是近海海洋生物不可避免的环境。随着海底电缆的大幅增加，电缆产生的磁场对海洋生物的影响逐渐引起人们的关注。然而,关于磁场对软体动物的影响的研究很少。探讨磁场是否会干扰近海软体动物的生理功能，在这里，我们系统地分析了暴露于地磁场或1.1T静磁场的Elysialeuolegnote的代谢和转录组的变化。血糖和血脂水平，暴露于高静磁场10天后，白油中的抗氧化酶活性也显着增加。同时,与消化性能和肝功能相关的酶活性降低。通过比较转录组分析进一步揭示了可能的机制。共鉴定出836个差异表达基因,暴露于高静磁场后，其中352个上调，484个下调。上调的差异基因主要集中在溶酶体和凋亡途径,下调的差异基因主要涉及消化和免疫系统，包括吞噬作用。通过RT-qPCR分析进一步证实了这种模式。总之,长期暴露于1.1T静磁场会增加氧化应激、血糖和血脂水平，和降低的免疫力和生理条件。我们在这里提供的数据提供了暴露于静磁场的E.leucolegnote的代谢变化和基因表达模式的全面视图。它可能会在分子水平上扩展我们对磁场对近海软体动物的影响的认识，并有助于澄清海洋动物与人造磁场之间的相互作用，这在生态上当然很重要。
