Despite the plethora of studies on the impacts of access to runs on chickens, there is a paucity of information on the welfare and behavioural repertoire of hens raised in the deep litter houses with or without access to legume- or grass-based pasture. Therefore, this study aimed to evaluate the impact of access to grass or legume pastures by laying hens on behaviour, physiological responses and bacterial load. The study was conducted to evaluate the influence of exposure of egg-type chickens to runs on grass or legume pastures on their welfare and behaviours. The study involved the use of 240 ISA brown pullets from 12 weeks of age and and lasted for 48 weeks. The treatments were deep litter housing with grass-based pasture run (PG), deep litter housing with legume-based pasture run (PL) and deep litter housing without runs (LD) having 80 pullets with four replicates of twenty birds each. Behavioural observations of the hens in each pen were made at 52 weeks of age and tonic immobility was assessed by making the birds lie on their back with their head resting in a U-shaped wooden cradle. The measurements of the respiratory rate and rectal temperature of the hens were assessed at 1:00 p.m. at different laying phases. The gastrointestinal and egg bacterial counts were conducted at 60 weeks of age. Results revealed that the proportion of time spent eating was highest (p < 0.05) in the deep litter housing system, while the legume and grass pasture were similar. The hens spent most of their time standing and eating in the three treatments. However, the time spent standing in PL and PG was similar but significantly higher (p < 0.05) than in LD. Results on tonic immobility duration showed that the time spent by the hens in LD during the reaction was significantly longer than those of the PL and PG in the first, second and third phases of the experiments. However, the time spent by the hens in PL and PG was similar. The rectal temperatures of PL and PG birds were comparable and higher than those of LD during the second phase. On the other hand, there was no difference in the respiratory rate. Plasma triiodothyronine (T3) of the hens did not follow a consistent pattern. The bacterial count in the large intestine in LD and PL was similar but significantly (P < 0.05) higher than that of the PG. It was concluded that access to pasture influenced the behaviours of hens and that tonic immobility duration was shorter in the hens on the pasture, suggesting that access to pasture favoured hens\' welfare.

The coronavirus (COVID-19) pandemic has caused financial hardship and psychological distress among young Australians. This study investigates whether the Australian Government\'s emergency cash transfer payments-specifically welfare expansion for those unemployed prior to the pandemic (known in Australia as the Coronavirus Supplement) and JobKeeper (cash support for those with reduced or stopped employment due to the pandemic)-were associated with individual\'s level of coping during the coronavirus pandemic among those with and without mental disorders (including anxiety, depression, ADHD and autism). The sample included 902 young adults who participated in all of the last three waves (8, 9C1, 9C2) of the Longitudinal Study of Australian Children (LSAC), a nationally representative cohort study. Modified Poisson regression models were used to assess the impact of emergency cash transfer payments on 18-22-year-old\'s self-rated coping level, stratifying the analysis by those with and without mental disorders. All models were adjusted for gender, employment, location, family cohesion, history of smoking, alcohol intake, and COVID-19 test result. Of the 902-person sample analysed, 41.5% (n = 374) reported high levels of coping, 18.9% (n = 171) reported mental disorders, 40.3% (n = 364) received the Coronavirus Supplement and 16.4% (n = 148) received JobKeeper payments. Analysing the total sample demonstrated that participants who received the JobKeeper payment were more likely to have a higher level of coping compared to those who did not receive the JobKeeper payment. Stratified analyses demonstrated that those with pre-existing mental disorder obtained significant benefit from the JobKeeper payment on their level of coping, compared to those who did not receive JobKeeper. In contrast, receipt of the Coronavirus Supplement was not significantly associated with higher level of coping. Among those with no mental health disorder, neither the Coronavirus Supplement nor JobKeeper had a statistically significant impact on level of coping. These findings suggest the positive impacts of cash transfers on level of coping during the pandemic were limited to those with a pre-existing mental disorder who received JobKeeper.

Single-confinement housing can pose welfare risks to domestic horses. This study investigated horses\' stress responses when confined to single stalls in different stable designs in a tropical savanna region to address a gap in the literature. In total, 23 horses were assigned to a stable with a central corridor and solid external walls (A) (N = 8), a stable with one side corridor and solid external walls (B) (N = 6), or a stable with a central corridor and no solid external walls (C) (N = 9). Air velocity, relative humidity, air temperature, and noxious gases were measured inside the stables, and the heart rate and HRV of the horses were also determined. The relative humidity was lower in stable C than in stable A (p < 0.05), while the air temperature was higher in stable C than in stable B (p < 0.05) during the day. The airflow and ammonia levels were higher in stable C than in stables B and A (p < 0.01-0.0001). Overall, horses\' HRV in stable A was lower than in those in stables B and C (p < 0.05-0.01). Horses in stable A tended to experience more stress than those in other stables.

The aim of the present study was to assess the short-term behavioral and physiological responses of piglets to different treatment protocols for the control of iron-deficiency anemia (IDA) and cystoisosporosis. Piglets were treated with either (1) an injection of iron combined with an oral application of toltrazuril (TLZ) by drenching or (2) a combination injection of TZL + gleptoferron; the behavior of the piglets was then evaluated. For this study, 288 piglets were divided into three experimental groups: 96 piglets were kept untreated (control group); 96 piglets received an oral administration of a generic TZL-based anticoccidial agent (20 mg/kg BW) along with intramuscular administration of iron dextran (200 mg/mL; 1 mL/piglet) at the same handling (oral + parenteral group, O + P); and 96 piglets received an intramuscular application of the combination product (parenteral group, P). For each treated piglet, the total handling time, flight reaction, and the intensity and frequency of vocalizations were determined using the methodology described by Scollo et al. (2020). Piglets in the O + P group were found to emit more screams during treatment administration than animals in the P group (21.05% vs. 8.42% of animals; p < 0.05). Piglets in the O + P group reacted worse to manipulation and oral administration because a higher percentage of animals continued to fidget even after handling (32.63% vs. 12.63%; p < 0.05). Differences in growth performance between the groups were not observed in our study (p > 0.05). In conclusion, the administration of a combination product reduced stress during administration, as indicated by reduced vocalizations and reactions to manipulation.

This study investigated the effects of lairage conditions on the welfare and meat quality of pigs during lairage periods. A total of 3070 finishing pigs were assigned to one of six groups arranged in two trials in a 2 × 3 factorial design according to the illuminance (under 40 lux (LX), over 40 lux (HX)) and stocking density (low density (LD), higher than 0.83 m2/100 kg; normal density (ND), 0.50-0.83 m2/100 kg; high density (HD), lower than 0.50 m2/100 kg) with high temperature (HT), higher than 24 °C; low temperature (LT), lower than 10 °C. Pigs stocked with HD showed lower aggression behavior and overlap behavior than those stocked with LD at LT. Pigs stocked with HD showed higher standing, sitting, and aggression behavior than those stocked with LD at HT. Pigs stocked with HD showed higher pH than those stocked with LD at LT. At HT, pigs stocked with LD showed higher pH, WHC, DL, and CL than those stocked with HD. At LT, pigs stocked with LD showed higher cortisol levels than those stocked with HD. However, pigs stocked with LD showed lower cortisol levels than those stocked with HD at HT. Based on the obtained results, stocking of too-high (lower than 0.50 m2/100 kg) density at HT and stocking of too-low (higher than 0.83 m2/100 kg) density at LT are generally not good for meat quality and animal welfare.

Greater knowledge is always an advantage for a rational individual. However, this article shows that for a group of rational individuals greater knowledge can backfire, leading to a worse outcome for all. Surprisingly, this can happen even when new knowledge does not mean the discovery of a new action but simply provides a deeper understanding of the interaction at stake. More specifically, enhanced knowledge about the current state of nature may hinder cooperation among purely self-interested individuals. The paper describes this paradoxical possibility-a \'knowledge curse\'-and analyses the evolutionary process that occurs if, initially, only a few people have access to the greater knowledge. It concludes with a tentative comment on ways to avert this potential knowledge backlash.

BACKGROUND: The current study was conducted to assess the impact of different LED light colors on welfare indicators in Muscovy ducks. These welfare parameters encompassed growth performance, specific behaviors, tonic immobility (TI), feather score, haematological, and serum biochemical parameters. Eighty-four healthy unsexed Muscovy ducklings aged two weeks were randomly assigned to four groups (3replicates/group; each replicate contains 7 birds) based on different LED light colors. The first group was raised under white light, the second under red light, the third under blue light, and the fourth under yellow light. To assess the impact of various LED light colors on welfare, growth performance indicators (body weight, body weight gain, feed intake, and feed conversion ratio) were measured. Behavioral patterns including feeding, drinking, standing, walking, sitting, feather pecking, and other activities were recorded. Tonic immobility test (TI) and feather condition scoring were conducted at 3, 6, and 10 weeks of age. At the end of the study blood samples were collected for hematological and serum biochemical analyses.
RESULTS: The results revealed that using blue, yellow, and red colors had no adverse effect on the final body weight of the ducks (P > 0.05). Unlike to red light, blue light significantly reduced feather pecking, TI time and cortisol concentrations and improved the feather condition score (P ≤ 0.05).
CONCLUSIONS: The current findings suggest that the application of blue light effectively improves welfare indices and has no detrimental impact on the growth performance of Muscovy ducks thereby positively contributing to their welfare.

CRISPR-associated proteins and clustered regularly interspaced short palindromic repeats (CRISPR-Cas) technology has emerged as a groundbreaking advancement in animal and poultry nutrition to improve feed conversion efficiency, enhance disease resistance, and improve the nutritional quality of animal products. Despite significant advancements, there is a research gap in the systematic understanding and comprehensive use of the CRISPR-Cas method in animal and poultry nutrition. The purpose of this study is to elucidate the latest advancements in animal and poultry nutrition through CRISPR-Cas genome editing technology, focusing on gene manipulation in metabolism, immunity, and growth. Following preferred reporting items in meta-analysis and systematic reviews guidelines, we conducted a systematic search using several databases, including Scopus, PubMed, and Web of Science, until May 2024, and finally, we included a total of 108 articles in this study. This article explores the use of the CRISPR-Cas system in the advancement of feed additives like probiotics and enzymes, which could reduce the use of antibiotics in animal production. Furthermore, the article discusses ethical and regulatory issues related to gene editing in animal and poultry nutrition, including concerns about animal welfare, food safety, and environmental impacts. Overall, the CRISPR-Cas system holds substantial promise to overcome the challenges in modern animal agriculture. By enriching the nutritional quality of animal products, increasing disease resistance, and improving feed efficiency, it offers sustainable and cost-effective solutions that can revolutionize animal and poultry nutrition.

BACKGROUND: The healthcare system is under tremendous pressure. One possible solution towards relieving some of this pressure is to use Positive Health, which takes \'health\' as a starting point, rather than \'illness\'. Positive Health provides opportunities for stimulating integrated care.
METHODS: Three cases in the Netherlands are studied in this paper. Their way of working with Positive Health is investigated through semi-structured and narrative interviews, using realist-evaluation and thematic analyses.
RESULTS: Seven \'working elements\' are identified that enhance the chances of successfully implementing Positive Health in practice (part 1). The interviews show that healthcare professionals have noticed that people adopt a healthier lifestyle and gain a greater degree of control over their own health. This boosts job satisfaction for healthcare professionals too. The organisations and professionals involved are enthusiastic about working with Positive Health, but still experience barriers (part 2).
CONCLUSIONS: The results of this study imply that implementing Positive Health in practice can facilitate collaboration between organisations and professionals from different disciplines, such as healthcare, welfare, and municipal health services. Operating from the perspective of a shared goal, professionals from different disciplines will find it easier to jointly organise activities to foster citizens\' health. Additionally, more attention is paid to non-medical problems affecting people\'s well-being, such as loneliness or financial problems.

Most studies of the effects of housing and husbandry on animals\' affective states and welfare investigate the impact of stable living conditions, comparing for example, animals living in enriched environments with those living in non-enriched ones. Changes in living conditions, including from more to less enriched environments, have also been found to have effects on measures of affective state and welfare in some species. But these studies have not investigated whether it is the trajectory of change that has affected the animals (e.g., worsening conditions), or simply the nature of their final environment (e.g., non-enriched). Here, we hypothesised that laying hens living in worsening conditions across a six-week period (gradually moving from preferred to non-preferred living conditions; \"Trajectory to Non-Preferred\", TNP, n = 30), would show evidence of more negative affective states and poorer welfare than those living continuously in non-preferred conditions for the same duration (\"Stable Non-Preferred\", SNP, n = 30). We also hypothesised that hens living in improving conditions (gradually moving from non-preferred to preferred living conditions; \"Trajectory to Preferred\", TP, n = 30), would show evidence of more positive affective states and better welfare than those living continuously in preferred conditions (\"Stable Preferred\", SP, n = 30). The preferred living condition provided extensive resources and intermittent rewarding events (such as the delivery of food treats) known to be valued and preferred by most hens, while the non-preferred living condition provided just basic resources and intermittent aversive events (e.g., loud noises). The hens\' affective states and welfare were measured using home-pen behavioural observations, body condition assessments, physiological stress measures (e.g., blood corticosterone, glucose, etc.), physical challenge tests, and judgement bias tests. A number of differences between hens in the trajectory and stable living conditions were found: TP hens were lighter, showed more foraging behaviour and less standing alert and head-shaking than SP hens, while TNP hens showed more head-shaking, mild feather pecking and aggressive attacking of pen mates than SNP hens. However, some of these differences failed to reach significance following Benjamini adjustments for multiple testing. The groups also did not differ in their judgement biases (measured in a sub-sample of 12 hens per experimental group), response to physical challenges, or measures of physiological stress. We conclude that the hens in the present study showed some evidence of responsiveness to \'affective trajectories\' in their living conditions, but no definitive effects on their affective states and welfare.