这项研究的目的是研究apelin可能的保护作用,已知具有抗氧化和抗炎作用,L-NAME诱导子痫前期妊娠大鼠新生儿神经发生的变化。Wistar白化病雌性大鼠分为四个实验组:对照组,Apelin,先兆子痫和先兆子痫+Apelin。五号测量血压,妊娠的第11天和第17天,从6日收集的24小时尿液样本中分析尿蛋白,第12天和第18天,从血清样品中分析血清肌酐。取母体肾脏和胎盘组织建立子痫前期模型,和新生儿的脑组织,包括皮质,获得海马和小脑区域以研究神经发生,并通过组织学和免疫组织化学方法进行检查。新生儿的数量,测量新生儿的体重和脑重量。eNOS,通过ELISA分析脑中的IL-10、nNOS和NO水平。平均动脉压,先兆子痫患者尿蛋白和血清肌酐升高。先兆子痫组新生儿体重减轻,子痫前期+Apelin组的值更接近对照组和Apelin组。先兆子痫组,肾脏近端和远端小管的水肿和扩张,观察到胎盘绒毛周围纤维蛋白沉积和合胞体结节增加。肾脏和胎盘中VEGF免疫反应性降低,iNOS免疫反应性增加。在新生儿脑组织检查中,细胞毒性水肿伴随皮质变薄,海马中的迁移延迟和较低的细胞计数,在先兆子痫中观察到小脑细胞间隙增加和EGL增厚。NeuN的表达,GFAP,MBP,IL-10eNOS,nNOS和NO水平下降,而Iba-1的表达在先兆子痫中增加。在先兆子痫+Apelin组中,这些发现与对照组和Apelin组相似.发现给予Apelin有益于预防先兆子痫的不良后果。但是需要进一步的实验和临床研究来更好地了解这些影响。
The aim of this study was to investigate the possible protective effects of
apelin, which is known to have antioxidant and anti-inflammatory effects, on changes in neurogenesis in newborns of pregnant rats with L-NAME-induced preeclampsia. Wistar albino female rats were divided into four experimental groups: Control,
Apelin, Preeclampsia and Preeclampsia +
Apelin. Blood pressure was measured on the 5th, 11th and 17th days of gestation, urine protein was analyzed from urine samples collected for 24 h on the 6th, 12th and 18th days and serum creatinine was analyzed from serum samples. Maternal kidney and placenta tissues were obtained to establish the preeclampsia model, and neonatal brain tissues including the cortex, hippocampus and cerebellum regions were obtained to investigate neurogenesis and examined by histological and immunohistochemical methods. The number of newborns, body weight and brain weight of the newborns were measured. eNOS, IL-10, nNOS and NO levels in the brain analyzed via ELISA. Mean arterial pressure, urine protein and serum creatinine increased in the preeclampsia. Newborn weight decreased in the Preeclampsia group, the values in the Preeclampsia + Apelin group were closer to the Control and
Apelin groups. In the Preeclampsia group, edema and dilatation in the proximal and distal tubules of kidneys, perivillous fibrin deposition and increase in syncytial nodules of placenta were observed. VEGF immunoreactivity decreased and iNOS immunoreactivity increased in both kidney and placenta. In neonatal brain tissue examinations, cytotoxic edema accompanied by thinning of cortex, delayed migration and lower cell counts in the hippocampus, and increase in intercellular spaces and EGL thickening in the cerebellum were observed in the preeclampsia. Expression of NeuN, GFAP, MBP, IL-10, eNOS, nNOS and NO levels decreased, whereas expression of Iba-1 increased in the preeclampsia. In the Preeclampsia +
Apelin group, these findings were similar to the Control and
Apelin groups. Apelin administration was found to be beneficial for preventing the adverse consequences of preeclampsia, but further experimental and clinical studies are needed to better understand these effects.