Cu2+加速α-突触核蛋白原纤维的病毒样繁殖,并在帕金森病(PD)的发病机制中起关键作用。因此,Cu2+的准确检测对PD等神经系统疾病的诊断至关重要。Cu2+检测过程受到具有相似电化学性质的物质的阻碍。在这项研究中,石墨烯(GDY),一种新型的碳同素异形体,具有很强的电子供给能力,利用其对Cu2的出色吸附能力,用于Cu2的高选择性检测。密度泛函理论(DFT)计算表明Cu原子吸附在GDY的空腔中,Cu和C原子之间的吸收能高于石墨烯(GR),说明GDY的空穴有利于Cu原子的吸附和电化学传感。基于GDY的电化学传感器可以有效避免氨基酸的干扰,金属离子和神经递质,具有9.77μA·μM-1·cm-2的高灵敏度,最小可检测浓度为200nM。在以α-突触核蛋白为诊断标准的PD发病机制和治疗过程中,检测L-DOPA和GSH处理前后细胞中Cu2+的浓度,发现Cu2+作为PD的生物标志物具有很高的潜力。本研究不仅利用GDY和Cu2+的良好吸附来提高离子检测的特异性,而且为更深入地理解Cu2+在神经生物学和神经系统疾病中的作用提供线索。
Cu2+ accelerates the viral-like propagation of α-synuclein fibrils and plays a key role in the pathogenesis of Parkinson\'s disease (PD). Therefore, the accurate detection of Cu2+ is essential for the diagnosis of PD and other neurological diseases. The Cu2+ detection process is impeded by substances that have similar electrochemical properties. In this study,
graphdiyne (GDY), a new kind of carbon allotrope with strong electron-donating ability, was utilized for the highly selective detection of Cu2+ by taking advantage of its outstanding adsorption capacity for Cu2+. Density functional theory (DFT) calculations show that Cu atoms are adsorbed in the cavity of GDY, and the absorption energy between Cu and C atoms is higher than that of graphene (GR), indicating that the cavity of GDY is favorable for the adsorption of Cu atoms and electrochemical sensing. The GDY-based electrochemical sensor can effectively avoid the interference of amino acids, metal ions and neurotransmitters and has a high sensitivity of 9.77 μA·μM-1·cm-2, with a minimum detectable concentration of 200 nM. During the investigating pathogenesis and therapeutic process of PD with α-synuclein as the diagnostic standard, the concentration of Cu2+ in cells before and after L-DOPA and GSH treatments were examined, and it was found that Cu2+ exhibits high potential as a biomarker for PD. This study not only harnesses the favorable adsorption of the GDY and Cu2+ to improve the specificity of ion detection but also provide clues for deeper understanding of the role of Cu2+ in neurobiology and neurological diseases.