Abstract：
Photothermal modulation of neural activity offers a promising approach for understanding brain circuits and developing therapies for neurological disorders. However, the low neuron selectivity and inefficient light-to-heat conversion of existing photothermal nanomaterials significantly limit their potential for neuromodulation. Here, we report that graphdiyne (GDY) can be developed into an efficient neuron-targeted photothermal transducer for in vivo modulation of neuronal activity through rational surface functionalization. We functionalize GDY with polyethylene glycol (PEG) through noncovalent hydrophobic interactions, followed by antibody conjugation to specifically target the temperature-sensitive transient receptor potential cation channel subfamily V member 1 (TRPV1) on the surface of neural cells. The nanotransducer not only exhibits high photothermal conversion efficiency in the near-infrared region but also shows great TRPV1-targeting capability. This enables photothermal activation of TRPV1, leading to neurotransmitter release in cells and modulation of neural firing in living mice. With its precision and selectivity, the GDY-based transducer provides an innovative avenue for understanding brain function and developing therapeutic strategies for neurodegenerative diseases.
摘要：
神经活动的光热调制为了解大脑回路和开发神经系统疾病的疗法提供了一种有前途的方法。然而,现有光热纳米材料的低神经元选择性和低效率的光热转换显著限制了其神经调节的潜力。这里,我们报告说，石墨烯（GDY）可以发展成为一种有效的神经元靶向光热换能器，用于通过合理的表面功能化在体内调节神经元活动。我们通过非共价疏水相互作用用聚乙二醇（PEG）官能化GDY，然后通过抗体偶联特异性靶向神经细胞表面的温敏瞬时受体电位阳离子通道亚家族V成员1(TRPV1)。纳米换能器不仅在近红外区域表现出高的光热转换效率，而且还表现出很大的TRPV1靶向能力。这使得TRPV1能够光热激活,导致细胞中的神经递质释放和活小鼠中的神经放电的调节。凭借其精密度和选择性，基于GDY的换能器为了解脑功能和开发神经退行性疾病的治疗策略提供了创新途径.
