硬膜缺损和随后的脑脊液(CSF)泄漏通常出现在创伤或神经外科手术中,并伴随一系列严重的并发症甚至死亡。使用具有多功能泄漏阻塞功能的合格硬脑膜替代品,防粘连,硬脑膜重建是有前途的治疗方法之一。然而,尽管有些产品已经在诊所使用,没有一个替代品实现了所需的多功能。在这项研究中,我们旨在设计和制造具有理想多功能的硬脑膜修复复合材料。通过生物模拟天然硬脑膜的结构和成分,我们应用聚(L-乳酸)(PLLA),壳聚糖(CS),明胶,和脱细胞小肠粘膜下层(SIS)粉末成功制备了三层复合材料。然后,开发了一系列特定的设备和技术来研究性能。结果表明,在组分之间良好的协同作用下,可以实现令人满意的结构稳定性。此外,所有研究结果表明,仿生三层复合材料显示出令人满意的渗漏阻断多功能,防粘连,抗菌性能,和硬脑膜重建潜力,因此,它可能是硬脑膜修复的有希望的候选人。重要声明:开发具有多功能渗漏阻断功能的合格硬脑膜替代品,防粘连,硬脑膜重建对于治疗创伤或神经外科手术中出现的硬脑膜缺损和随后的脑脊液(CSF)泄漏至关重要。在这项研究中,基于天然硬脑膜的结构和成分的生物模拟,我们设计并制造了三层硬脑膜修复生物复合材料,该复合材料具有令人满意的结构稳定性和所需的多功能。此外,开发了一系列特定的设备和技术来研究相关性能。总的来说,开发的水凝胶静电纺丝体系在实现多功能方面表现出优异的优势,未来可广泛应用于实现多功能组织修复材料。
Dura mater defect and subsequent cerebrospinal fluid (CSF) leakage usually appear in trauma or neurosurgical procedures and are followed by a series of serious complications and even death. The use of a qualified dura mater substitute with multifunction of leakage blockade, adhesion prevention, and dura reconstruction is one of the promising treatment methods. However, even though some products have been used in the clinic, none of the substitutes achieved the required multifunction. In this study, we aimed to design and fabricate a dura repair composite with the ideal multifunction. By biomimicking the structure and component of natural dura, we applied poly(L-lactic acid) (PLLA), chitosan (CS), gelatin, and acellular small intestinal submucosa (SIS) powders to successfully prepare a triple-layered composite. Then, a series of specific devices and techniques were developed to investigate the performance. The results revealed that satisfactory structural stability could be realized under good synergistic interactions among the components. In addition, all the findings suggested that the bionic triple-layered composite showed satisfactory multifunction of leakage blockade, adhesion prevention, antibacterial property, and dura reconstruction potential, and thus, it might be a promising candidate for dura repair. STATEMENT OF SIGNIFICANCE: Developing qualified dura mater substitutes with multifunction of leakage blockade, adhesion prevention, and dura reconstruction is crucial for treating dura mater defect and subsequent cerebrospinal fluid (CSF) leakage that appear in trauma or neurosurgical procedures. In this study, we designed and fabricated a triple-layered dura repair biocomposite with satisfactory structural stability and desired multifunction based on biomimicking of the structure and component of natural dura. Moreover, a series of specific devices and techniques were developed to investigate the relevant performance. Overall, the developed hydrogel electrospinning system exhibited excellent advantages in achieving multifunction and could be applied widely in the future to achieve multifunctional tissue repair materials.