Abstract：
Postoperative adhesion is a noteworthy clinical complication in abdominal surgery due to the existing physical barriers are unsatisfactory and inefficient in preventing its occurrence. In this work, an elaborate nanoparticle-in-microgel system (nMGel) is presented for postoperative adhesion prevention. nMGel is facilely formed by crosslinking manganese dioxide (MnO2) nanoparticles-loaded gelatin microspheres with polydopamine using a modified emulsification-chemical crosslinking method, generating a nano-micron spherical hydrogel. After drying, powdery nMGel with sprayability can perfectly cover irregular wounds and maintains robust tissue adhesiveness even in a wet environment. Additionally, nMGel possesses prominent antioxidant and free radical scavenging activity, which protects cell viability and preserves cell biological functions in an oxidative microenvironment. Furthermore, nMGel displays superior hemostatic property as demonstrated in mouse tail amputation models and liver trauma models. Importantly, nMGel can be conveniently administrated in a mouse cecal defect model to prevent adhesion between the injured cecum and the peritoneum by reducing inflammation, oxidative stress, collagen synthesis, and angiogenesis. Thus, the bioactive nMGel offers a practical and efficient approach for ameliorating postsurgical adhesion.
摘要：
术后粘连是腹部手术中值得注意的临床并发症，因为现有的物理屏障不能令人满意且预防其发生效率低下。在这项工作中,提出了一种精细的纳米颗粒微凝胶系统（nMGel）用于预防术后粘连。nMGel是通过使用改进的乳化-化学交联方法将载有二氧化锰（MnO2）纳米颗粒的明胶微球与聚多巴胺交联而容易形成的，产生纳米微米球形水凝胶。干燥后,具有喷雾性的粉状nMGel可以完美地覆盖不规则的伤口，即使在潮湿的环境中也能保持强大的组织粘附性。此外,NMGel具有突出的抗氧化和自由基清除活性，在氧化微环境中保护细胞活力并保持细胞生物学功能。此外，nMGel显示出如在小鼠尾部截肢模型和肝创伤模型中所证明的优越的止血性质。重要的是,nMGel可以方便地在小鼠盲肠缺损模型中施用，以通过减少炎症来防止损伤的盲肠和腹膜之间的粘连。氧化应激，胶原蛋白合成，和血管生成。因此，生物活性nMGel为改善术后粘连提供了一种实用有效的方法.
