Abstract：
The development of anti-adhesion hydrogels for preventing postoperative adhesions is an ongoing challenge, particularly in achieving a balance between exceptional antifouling properties and effective in situ tissue retention. In this study, we propose a unique approach with the design of a single-component Janus zwitterionic hydrogel patch featuring a bionic microstructure. The Janus patches were prepared through free radical polymerization of sulfobetaine methacrylate with N, N\'-methylenebis(2-propenamide) as the cross-linker. The incorporation of hexagonal facets separated by interconnecting grooves on one side imparts durable and reliable in situ retention capabilities to the Janus hydrogel patch when it is applied to traumatized tissues. The opposing flat surface exhibits outstanding resistance to bacteria, proteins, and cell adhesion, due to the superhydrophilicity and excellent antifouling characteristics of zwitterionic polymers. This dual functionality empowers the Janus hydrogel patch to mitigate adhesions between traumatized and surrounding tissues. The hexagonal and groove bionic microstructures facilitate rapid drainage, promoting swift contact with the tissue for increased adhesion strength, while independent hexagonal microfacets enhance the peeling energy. In an in vivo setting, Janus zwitterionic hydrogel patches with surface microstructures form mutually embedded structures with the cecum surface, minimizing the likelihood of slippage and detachment. Remarkably, in vivo experiments involving abdominal wall cecum injuries illustrate the Janus zwitterionic hydrogel patch\'s superior anti-adhesion effectiveness compared to commercial controls. Thus, the Janus hydrogel patch, distinguished by its bionic microstructure surface, presents substantial potential in the biomedical field for averting postoperative adhesions.
摘要：
开发用于预防术后粘连的抗粘连水凝胶是一个持续的挑战，特别是在实现优异的防污性能和有效的原位组织保留之间的平衡。在这项研究中,我们提出了一种独特的方法，设计了具有仿生微结构的单组分Janus两性离子水凝胶贴片。Janus贴剂是通过磺基甜菜碱甲基丙烯酸酯与N，N'-亚甲基双(2-丙烯酰胺)作为交联剂。当Janus水凝胶贴片应用于受创伤的组织时，通过在一侧互连凹槽分开的六边形小平面的结合为其提供了持久且可靠的原位保留能力。相对的平坦表面对细菌表现出突出的抵抗力，蛋白质，和细胞粘附，由于两性离子聚合物的超亲水性和优异的防污特性。这种双重功能使Janus水凝胶贴片能够减轻创伤和周围组织之间的粘连。六边形和凹槽仿生微结构有利于快速排水，促进与组织的快速接触，以增加粘附强度，而独立的六边形微面增强剥离能量。在体内环境中，具有表面微结构的Janus两性离子水凝胶贴片与盲肠表面形成相互嵌入的结构，最大限度地减少打滑和脱离的可能性。值得注意的是,涉及腹壁盲肠损伤的体内实验表明，与商业对照相比，Janus两性离子水凝胶贴片具有优异的抗粘连效果。因此，Janus水凝胶贴片,以其仿生微结构表面而著称，在生物医学领域为避免术后粘连提供了巨大的潜力。
