Abstract：
Anastomosis surgery at the intestinal site is performed on millions of individuals every year. However, several persistent complications, such as anastomotic leakage, abnormal adhesion, and anastomotic stenosis, have been observed after the surgery. For promoting anastomotic healing and to overcome the challenges mentioned above, re-epithelialization at anastomotic sites is crucial. In this study, an epithelialization-promoting macromolecular prodrug Ala-Gln-PPDO was prepared and processed into fibrous membranes by electrospinning. Ala-Gln and gentamicin were sustainably released from the electrospun membranes with degradation of these membranes to promote the proliferation of rat intestinal epithelial cells and suppress the proliferation of Staphylococcus aureus and Escherichia coli. The comprehensive repair effects of Ala-Gln-PPDO membranes have been evaluated in rat models of intestinal anastomosis in this study. Application of Ala-Gln-PPDO membranes, especially the gentamicin-incorporated Ala-Gln-PPDO ones, could prevent adhesion between the injured intestine and surrounding intestinal tissues. In addition, they did not affect the healing strength of anastomotic stoma negatively and could promote re-epithelialization at the anastomotic sites. Furthermore, the gentamicin-incorporated Ala-Gln-PPDO membranes could relieve stenosis at anastomotic sites. The gentamicin-incorporated Ala-Gln-PPDO electrospun membrane is a promising, comprehensive implantable material for promoting healing after gastrointestinal anastomosis owing to its effects involving the promotion of re-epithelialization, prevention of adhesion, and relieving of anastomotic stenosis.
摘要：
每年对数百万人进行肠道部位的吻合手术。然而,几个持续的并发症，如吻合口漏，异常粘连,吻合口狭窄,手术后观察到了。为了促进吻合口愈合并克服上述挑战，吻合部位的再上皮化是至关重要的。在这项研究中,制备了一种促进上皮化的大分子前药Ala-Gln-PPDO，并通过静电纺丝加工成纤维膜。Ala-Gln和庆大霉素从静电纺丝膜中持续释放，并降解这些膜，以促进大鼠肠上皮细胞的增殖并抑制金黄色葡萄球菌和大肠杆菌的增殖。本研究已在大鼠肠吻合模型中评估了Ala-Gln-PPDO膜的综合修复作用。Ala-Gln-PPDO膜的应用,尤其是庆大霉素掺入的Ala-Gln-PPDO，可以防止受伤的肠道和周围肠道组织之间的粘连。此外,它们不会对吻合口的愈合强度产生负面影响，并且可以促进吻合部位的再上皮化。此外，庆大霉素掺入的Ala-Gln-PPDO膜可以缓解吻合口狭窄。庆大霉素掺入Ala-Gln-PPDO电纺膜是一种很有前途的,促进胃肠吻合术后愈合的综合性可植入材料由于其涉及促进再上皮化的作用，防止粘连，并缓解吻合口狭窄。
