在这项研究中,土壤培养6周后的小白菜幼苗用不同时间叶面施用TiO2NPs(20mg/L)。转录组学分析用于研究TiO2NP对生理学的影响,增长,结果表明,TiO2NPs的暴露显著增加了生物量,总磷,过氧化氢酶活性分别为23.60、23.72和44.01%,分别,与未处理的(不是散装或离子)相比。TiO2NPs使叶片叶绿素含量提高4.9%,光合速率提高16.62%,这归因于七个基因的上调表达(PetH,PetF,PsaF,PsbA,PsbB,PsbD,和Lhcb)与光系统I中的电子传输和叶片中的光捕获有关。与19种水通道蛋白基因表达的改变相关的水分平衡得到了改善(例如,PIP2;1和NIP6;1)。58个与植物激素信号和生长相关的基因表达失调,随着GA20、SnRK2和PP2C的显著下调和DELLA的上调,SAM,和ETR。此外,11个三羧酸循环基因和13个糖酵解基因似乎刺激了参与促进中国芽孢杆菌生长和生理的途径。这项研究为提高黄连产量的新策略提供了宝贵的见解。
In this study, Brassica chinensis L seedlings after 6 weeks of soil cultivation were treated with foliar application of TiO2
NPs (20 mg/L) for different times. Transcriptomics analysis was employed to investigate the impact of TiO2
NPs on the physiology, growth, and yield of B. chinensis L. Results showed that TiO2
NPs\' exposure significantly increased the biomass, total phosphorus, and catalase enzyme activity by 23.60, 23.72, and 44.01%, respectively, compared to the untreated ones (not bulk or ion).TiO2
NPs increased the leaf chlorophyll content by 4.9% and photosynthetic rate by 16.62%, which was attributed to the upregulated expression of seven genes (PetH, PetF, PsaF, PsbA, PsbB, PsbD, and Lhcb) associated with electron transport in photosystem I and light-harvesting in leaves. The water balance of B. chinensis was improved correlating with the altered expressions of 19 aquaporin genes (e.g., PIP2;1 and NIP6;1). The expressions of 58 genes related to plant hormone signaling and growth were dysregulated, with notable downregulations in GA20, SnRK2, and PP2C and upregulations of DELLAs, SAM, and ETR. Moreover, the 11 tricarboxylic acid cycle genes and 13 glycolysis genes appear to stimulate pathways involved in promoting the growth and physiology of B. chinensis. This research contributes valuable insights into new strategies for increasing the yield of B. chinensis.