Abstract：
Nanoplastics have now become a pervasive contaminant, being detected in various environmental media. However, our understanding of the specific toxicological effects of nanoplastics (NPs) on the kidneys remains unclear, which is a scientific problem that needs to be solved. To address this question, we employed two kidney cell lines as in vitro models to study the toxicological effects of NPs on porcine kidney cells. Firstly, we observed that NPs can be internalized into the cytoplasm in a time- and dose-dependent manner by using a laser confocal microscope. We further discovered that NPs can trigger inflammatory responses and lead to porcine kidney cell senescence by detection of senescence marker molecules. Furthermore, the potential molecular mechanism(s) by which NPs induce porcine kidney cell senescence were explored, we found that NPs induce oxidative stress in the porcine kidney cells, leading to the accumulation of reactive oxygen species (ROS) within mitochondria, ultimately triggering inflammatory responses and senescence in the kidney cells. In summary, our experimental results not only provide new evidence for the toxicity of NPs but also offer new ideas and directions for future research. This discovery will aid in our deeper understanding of the potential health impacts of NPs on domestic pigs.
摘要：
纳米塑料现在已经成为一种普遍的污染物，在各种环境媒体中被检测到。然而,我们对纳米塑料（NPs）对肾脏的具体毒理学影响的理解仍不清楚，这是一个需要解决的科学问题。为了解决这个问题，我们采用两种肾细胞系作为体外模型来研究NPs对猪肾细胞的毒理学作用。首先,我们使用激光共聚焦显微镜观察到NPs可以以时间和剂量依赖性的方式内化到细胞质中.我们进一步发现，通过检测衰老标记分子，NP可以触发炎症反应并导致猪肾细胞衰老。此外，探讨了NPs诱导猪肾细胞衰老的潜在分子机制,我们发现NPs在猪肾细胞中诱导氧化应激，导致线粒体内活性氧（ROS）的积累，最终引发肾细胞的炎症反应和衰老。总之,实验结果不仅为NPs的毒性研究提供了新的证据,也为今后的研究提供了新的思路和方向。这一发现将有助于我们更深入地了解NPs对家猪的潜在健康影响。
