目的:平面细胞极性(PCP)信号,对于活动纤毛的均匀排列和定向跳动至关重要,已在多纤毛上皮中进行了研究。作为连接中耳和鼻咽的复杂结构,咽鼓管(ET)在耳鼻喉疾病的发作中很重要。然而,PCP信令,包括对ET中的纤毛运动和清除功能很重要的方向,没有被研究过。我们评估了ET上皮中的PCP。
方法:小鼠ET的形态测量检查。
方法:我们进行了电子显微镜检查,以评估小鼠ET的纤毛极性,随着免疫组织化学分析PCP蛋白在ET上皮中的定位。
结果:我们在ET上皮中发现了PCP。在单个和相邻细胞中,活动纤毛沿相同方向排列;这种排列在多纤毛细胞中表现为纤毛极性。此外,PCP蛋白不对称地位于ET平面中的相邻细胞之间。
结论:多纤毛ET上皮表现出极化,提示可能对ET功能至关重要的新结构特征。
方法:NA喉镜,2024.
OBJECTIVE: Planar cell polarity (PCP) signaling, essential for uniform alignment and directional beating of motile cilia, has been investigated in multiciliated epithelia. As a complex structure connecting the middle ear to the nasopharynx, the eustachian tube (ET) is important in the onset of ear-nose-throat diseases. However, PCP signaling, including the orientation that is important for ciliary motility and clearance function in the ET, has not been studied. We evaluated PCP in the ET epithelium.
METHODS: Morphometric examination of the mouse ET.
METHODS: We performed electron microscopy to assess ciliary polarity in the mouse ET, along with immunohistochemical analysis of PCP protein localization in the ET epithelium.
RESULTS: We discovered PCP in the ET epithelium. Motile cilia were aligned in the same direction in individual and neighboring cells; this alignment manifested as ciliary polarity in multiciliated cells. Additionally, PCP proteins were asymmetrically localized between adjacent cells in the plane of the ET.
CONCLUSIONS: The multiciliated ET epithelium exhibits polarization, suggesting novel structural features that may be critical for ET function.
METHODS: NA Laryngoscope, 134:3795-3801, 2024.