Abstract：
Ribosomal RNA (rRNA) plays a vital role in binding amino acids together, which dictates the primary structure of a protein. Visualization of its intracellular distribution and dynamics during protein synthesis enables a better understanding of the correlated biological essence. However, appropriate tools targeting live cell rRNA that are capable of multimodal imaging at the nanoscale are still lacking. Here, we rationally designed a series of terpyridine ammonium iridium(III) complexes, one of which is capable of selectively labeling rRNA in living cells. Its metal core and photostable nature allow further super-resolution STED imaging of rRNA found on the rough endoplasmic reticulum at a ∼40 nm resolution that is well correlated under correlative light and electron microscopy (CLEM). Interestingly, the Ir(III) complex demonstrated rRNA dynamics in living cells while boosting protein synthesis at the nanoscale. Our work offers a versatile tool to visualize rRNA synchronously under optical and electron microscopy, which provides a better understanding of rRNA evolution in living systems.
摘要：
核糖体RNA（rRNA）在氨基酸结合中起着至关重要的作用，这决定了蛋白质的一级结构。蛋白质合成过程中其细胞内分布和动力学的可视化可以更好地理解相关的生物学本质。然而,仍然缺乏能够在纳米尺度进行多模态成像的靶向活细胞rRNA的适当工具.这里,我们合理设计了一系列三联吡啶铵铱（III）配合物，其中一种能够选择性标记活细胞中的rRNA。它的金属核心和光稳定性质允许在粗面内质网上发现的rRNA的进一步超分辨率STED成像，分辨率约为40nm，在相关的光和电子显微镜（CLEM）下具有良好的相关性。有趣的是,Ir(III)复合物显示了活细胞中的rRNA动力学,同时在纳米尺度上促进蛋白质合成.我们的工作提供了一种多功能工具，可以在光学和电子显微镜下同步可视化rRNA，这提供了对生物系统中rRNA进化的更好理解。
