这项研究是为了确定甘油单月桂酸酯和肉桂醛(GCM)补充的混合物对肠道形态的影响,豁免权,蛋鸡的抗氧化状态和盲肠菌群。14周龄健康蛋鸡(Jingfen-1株)1,120只,随机分为4组,每组10个重复28层,各层饲喂含0的日粮(对照组),或250、500和1,000mg/kgGCM,持续12周。结果表明,饲粮添加GCM显著增加肠绒毛高度和绒毛高度/隐窝深度,十二指肠绒毛区,肝脏和空肠中的总超氧化物二尿酶活性,空肠谷胱甘肽过氧化物酶活性,而十二指肠和空肠隐窝深度降低,28周龄蛋鸡肝脏中过氧化氢含量和空肠丙二醛含量(P<0.05)。同时,添加GCM可显著增加20、24和28周龄蛋鸡血清免疫球蛋白A和免疫球蛋白M浓度(P<0.05)。此外,在16SrRNA测序中观察到,添加GCM提高了蛋鸡肠道菌群的丰度和多样性。各组的优势细菌是门水平的类杆菌和厚壁菌,类杆菌和乳酸杆菌是优势属。在蛋鸡日粮中添加GCM改变了盲肠微生物区系的组成和结构。总之,添加GCM(500-1,000mg/kg饮食)可以改善肠道形态,免疫功能,蛋鸡的肠道和肝脏抗氧化状态和肠道菌群,从而提高肠道消化和吸收能力。这些发现为进一步探索GCM改善肠道健康的机制提供了新的途径。
This study was to determine the effects of the mixture of glycerol monolaurate and cinnamaldehyde (GCM) supplementation on the intestinal morphology, immunity, antioxidant status and cecal microbiota of laying hens. A total of 1,120 healthy laying hens (Jingfen-1 strain) at the age of 14 wk were randomly divided into 4 groups with 10 replicates of 28 layers in each and layers were fed diets containing 0 (control group), or 250, 500, and 1,000 mg/kg GCM for 12 wk. The results showed that dietary supplementation with GCM significantly increased intestinal villus height and villus height/crypt depth, duodenal villus area, total superoxide disumutase activities in the liver and jejunum, jejunal glutathione peroxidase activities while decreased duodenal and jejunal crypt depth, hydrogen peroxide content in the liver and jejunal malondialdehyde content of laying hens aging 28 wk (P < 0.05). Meanwhile, GCM addition significantly increased serum immunoglobulin A and immunoglobulin M concentration of layers at the age of 20, 24, and 28 wk (P < 0.05). Moreover, it was observed in the 16S rRNA sequencing that the addition of GCM elevated the abundance and diversity of gut microbiota in laying hens. The predominant bacteria from each group were Bacteroidota and Firmicutes at the phylum level and Bacteroides and Lactobacillus were the dominant genera. The composition and structure of cecal microflora were changed by the addition of GCM to the diet of laying hens. In conclusion, the addition of GCM (500-1,000 mg/kg diet) can improve intestinal morphology, immune function, intestinal and liver antioxidant status and intestinal flora of laying hens, thereby improving intestinal digestion and absorption capacity. These findings provide a new way to further explore the mechanism of GCM improving intestinal health.