PDF(Pubmed)
Abstract：
The objective of this study was to evaluate the effects of different levels of glycerol monolaurate (GML) on laying performance, egg quality, antioxidant capacity, intestinal morphology and immune function in late-phase laying hens. A total of 480 Hy-Line Variety Brown hens (age 54 wk) were randomly assigned to 5 treatments: the control group (basal diet) and 4 GML groups (basal diet supplemented with 100, 200, 300, and 400 mg/kg GML). Each treatment consisted of 8 replicates with 12 hens each and the trial lasted for 8 wk. The results showed that dietary inclusion of GML increased the ADFI in the entire experimental period and the average egg weight in wk 5 to 8 and wk 1 to 8 of the experiment (linear, P < 0.05). Dietary GML addition linearly increased albumen height, Haugh unit and yolk color, and quadratically increased eggshell thickness (P < 0.05). The serum SOD activity, T-AOC and IgG concentrations in the 200 mg/kg GML group, and GSH-Px activity in 200 and 300 mg/kg GML groups were increased, while the MDA concentration in 200 and 300 mg/kg GML groups was decreased than those in the control group (P < 0.05). The jejunal villus height and villus height: crypt depth in 300 mg/kg GML group were higher than that in the control group (P < 0.05). The mRNA expression of TLR4, IL-1β and TNF-α in spleen and jejunum decreased with the increase of dietary GML concentration (linear, P < 0.05). In conclusion, dietary GML supplementation could improve egg quality, antioxidant capacity, intestinal morphology and immune function in late-phase laying hens, and dietary 300 mg/kg GML inclusion is suggested.
摘要：
这项研究的目的是评估不同水平的甘油单月桂酸酯（GML）对产蛋性能的影响，鸡蛋质量,抗氧化能力，后期蛋鸡的肠道形态和免疫功能。将480只Hy-Line品种棕色母鸡（54周龄）随机分配到5个处理组：对照组（基础饮食）和4个GML组（基础饮食中添加100、200、300和400mg/kgGML）。每种处理包括8个重复,每个12只母鸡,并且试验持续8周。结果表明，饮食中包含GML会增加整个实验期间的ADFI以及实验的第5至8周和第1至8周的平均蛋重（线性，P<0.05)。膳食GML添加线性增加蛋白高度，Haugh单位和蛋黄颜色，蛋壳厚度呈二次增加(P<0.05)。血清SOD活性,200mg/kgGML组中的T-AOC和IgG浓度，200和300mg/kgGML组的GSH-Px活性增加，200和300mg/kgGML组MDA浓度较对照组降低(P<0.05)。300mg/kgGML组空肠绒毛高度和绒毛高度：隐窝深度均高于对照组（P<0.05）。脾脏和空肠TLR4、IL-1β和TNF-α的mRNA表达随着饲粮GML浓度的增加而降低(线性,P<0.05)。总之,膳食补充GML可以提高鸡蛋品质,抗氧化能力，后期蛋鸡的肠道形态和免疫功能，建议饮食中包含300mg/kg的GML。
