Abstract：
In this study, based on the discovery of thymol/glycerol monolaurate (GML) eutectic solvent, we studied the effect of GML as a multi-functional component (ripening inhibitor and antibacterial agent) on the formation, stability and antibacterial activity of eutectic nanoemulsions, and investigated the preservation of nanoemulsion in fresh pork. These results indicated that the formation of eutectic solvent was due to the hydrogen bonding between thymol and GML in the molten state. And eutectic nanoemulsions prepared with medium GML concentrations (20%, 40%, and 60%) of eutectic solvents as oil phases had small droplet diameters (<150 nm), exhibited sustained-release characteristics, and had excellent physicochemical stability. Moreover, the addition of GML enhanced the antibacterial activity of thymol nanoemulsion against S. aureus. as seen by their ability to inhibit affect formation more effectively. Treatment of fresh pork with optimized eutectic nanoemulsions (40% thymol/60% GML) extended its shelf life during refrigeration, which was mainly attributed to the ability of the encapsulated essential oil to inhibit microbial growth and lipid oxidation. These results provide a novel strategy to control Ostwald ripening and maintain the high antibacterial activity of thymol in nanoemulsion-based delivery systems.
摘要：
在这项研究中,基于百里酚/单月桂酸甘油酯（GML）共晶溶剂的发现，我们研究了GML作为多功能成分（催熟抑制剂和抗菌剂）对形成的影响，共晶纳米乳液的稳定性和抗菌活性，研究了纳米乳液在鲜猪肉中的保鲜效果。这些结果表明，低共熔溶剂的形成是由于百里酚和熔融状态下的GML之间的氢键。和用中等GML浓度(20%，40%,和60％）的共晶溶剂作为油相具有小的液滴直径（<150nm），表现出缓释特性，具有优异的物理化学稳定性。此外，GML的加入增强了百里酚纳米乳对金黄色葡萄球菌的抗菌活性。从它们更有效地抑制影响形成的能力可以看出。用优化的低共熔纳米乳液（40％百里酚/60％GML）处理新鲜猪肉，延长了冷藏期间的保质期，这主要归因于包封的精油抑制微生物生长和脂质氧化的能力。这些结果提供了控制奥斯特瓦尔德成熟和保持百里酚在基于纳米乳液的递送系统中的高抗菌活性的新策略。
