乙烯(ET)是调节植物生长的重要植物激素,发展和应激反应。乙烯不敏感3/乙烯不敏感3样(EIN3/EIL)转录因子家族,作为ET信号转导通路的关键调节因子,在调节ET反应基因的表达中起着重要作用。尽管EIN3/EIL家族成员的研究已经在许多物种中完成,它们在源自二倍体胡杨×P的另一种培养物中的双单倍体(DH)杨树中的作用。nigra(供体树,DT)仍然模棱两可。在这项研究中,在DH杨树基因组中总共鉴定出7个EIN3/EIL基因家族成员。对这些基因进行了基本的理化性质分析,这些蛋白质被预测定位于细胞核。根据系统发育关系,EIN3/EIL基因分为两组,同一组基因具有相似的基因结构和保守的基序。基于转录组数据分析了EIN3/EIL基因在不同DH杨树植物顶芽中的表达模式。同时,通过RT-qPCR检测了PsnEIL1,PsnEIN3,PsnEIL4和PsnEIL5基因在不同DH植物不同组织中的表达模式,包括顶端芽,幼叶,功能性叶子,木质部,形成层和根。以上呈现的发现表明PsnEIL基因在不同DH植物的各种组织中的表达水平的显著变化。最后,PsnEIL1基因在DT中过表达,转基因植物表现出矮化表型,说明PsnEIL1基因参与调控杨树的生长发育。在这项研究中,对DH杨树的EIN3/EIL基因家族进行了分析和功能鉴定,为今后探索EIN3/EIL基因功能提供了理论依据。
Ethylene (ET) is an important phytohormone that regulates plant growth, development and stress responses. The ethylene-insensitive3/ethylene-insensitive3-like (EIN3/EIL) transcription factor family, as a key regulator of the ET signal transduction pathway, plays an important role in regulating the expression of ET-responsive genes. Although studies of EIN3/EIL family members have been completed in many species, their role in doubled haploid (DH) poplar derived from another culture of diploid Populus simonii × P. nigra (donor tree, DT) remains ambiguous. In this study, a total of seven EIN3/EIL gene family members in the DH poplar genome were identified. Basic physical and chemical property analyses of these genes were performed, and these proteins were predicted to be localized to the nucleus. According to the phylogenetic relationship, EIN3/EIL genes were divided into two groups, and the genes in the same group had a similar gene structure and conserved motifs. The expression patterns of EIN3/EIL genes in the apical buds of different DH poplar plants were analyzed based on transcriptome data. At the same time, the expression patterns of PsnEIL1, PsnEIN3, PsnEIL4 and PsnEIL5 genes in different tissues of different DH plants were detected via RT-qPCR, including the apical buds, young leaves, functional leaves, xylem, cambium and roots. The findings presented above indicate notable variations in the expression levels of PsnEIL genes across various tissues of distinct DH plants. Finally, the PsnEIL1 gene was overexpressed in DT, and the transgenic plants showed a dwarf phenotype, indicating that the PsnEIL1 gene was involved in regulating the growth and development of poplar. In this study, the EIN3/EIL gene family of DH poplar was analyzed and functionally characterized, which provides a theoretical basis for the future exploration of the EIN3/EIL gene function.