Abstract：
CONCLUSIONS: The exploration and dissection of a set of QTLs and candidate genes for gray leaf spot disease resistance using two fully assembled parental genomes may help expedite maize resistance breeding. The fungal disease of maize known as gray leaf spot (GLS), caused by Cercospora zeae-maydis and Cercospora zeina, is a significant concern in China, Southern Africa, and the USA. Resistance to GLS is governed by multiple genes with an additive effect and is influenced by both genotype and environment. The most effective way to reduce the cost of production is to develop resistant hybrids. In this study, we utilized the IBM Syn 10 Doubled Haploid (IBM Syn10 DH) population to identify quantitative trait loci (QTLs) associated with resistance to gray leaf spot (GLS) in multiple locations. Analysis of seven distinct environments revealed a total of 58 QTLs, 49 of which formed 12 discrete clusters distributed across chromosomes 1, 2, 3, 4, 8 and 10. By comparing these findings with published research, we identified colocalized QTLs or GWAS loci within eleven clustering intervals. By integrating transcriptome data with genomic structural variations between parental individuals, we identified a total of 110 genes that exhibit both robust disparities in gene expression and structural alterations. Further analysis revealed 19 potential candidate genes encoding conserved resistance gene domains, including putative leucine-rich repeat receptors, NLP transcription factors, fucosyltransferases, and putative xyloglucan galactosyltransferases. Our results provide a valuable resource and linked loci for GLS marker resistance selection breeding in maize.
摘要：
结论：使用两个完全组装的亲本基因组探索和解剖一组QTL和灰斑病抗性候选基因可能有助于加快玉米抗性育种。玉米的真菌病被称为灰叶斑病（GLS），由玉米赤孢菌和玉米赤孢菌引起，在中国是一个重大问题，南部非洲,和美国。对GLS的抗性由具有加性效应的多个基因控制，并受基因型和环境的影响。降低生产成本的最有效方法是开发抗性杂种。在这项研究中,我们利用IBMSyn10加倍单倍体（IBMSyn10DH）种群来识别与多个位置的灰叶斑病（GLS）抗性相关的数量性状基因座（QTL）。对七个不同环境的分析显示，共有58个QTL，其中49个形成了分布在染色体1、2、3、4、8和10上的12个离散簇。通过将这些发现与已发表的研究进行比较，我们在11个聚类间隔内确定了共定位的QTL或GWAS基因座。通过整合转录组数据与亲本个体之间的基因组结构变异，我们共鉴定出110个基因,这些基因在基因表达和结构改变方面均表现出显著差异.进一步的分析揭示了19个潜在的候选基因编码保守的抗性基因结构域，包括推定的富含亮氨酸的重复受体，NLP转录因子，岩藻糖基转移酶，和推定的木葡聚糖半乳糖基转移酶。我们的研究结果为玉米GLS标记抗性选择育种提供了宝贵的资源和连锁位点。
