背景:在研究中,对生育酚的分析,使用从黄种和黑种品系之间的相互杂交的F1杂种获得的DH品系进行了plastochomanol-8和植物甾醇。
方法:用于研究的生物材料由两个DH系之间相互杂交的F1杂种获得的冬油油菜的两个DH种群组成:黄色和黑色种子。使用ColorFlex分光光度计测定种子颜色。通过脉冲NMR测定脂肪含量。生育酚的水平,和塑性色满醇-8使用HPLC分析。通过GC法测定植物甾醇的含量和组成。
结果:黑色种子亲本系的脂肪含量为49%,高于黄色种子亲本系的脂肪含量(44%)。DH系群体的脂肪含量范围为44%至51%。总生育酚含量为460至602mg/kg,α-T/γ-T比为0.66至1.09。在亲本系H2-26和Z-114中,总生育酚含量为534和525mg/kg,但α-T/γ-T比值分别为0.81和1.21。黄色种子的亲本系(Z-114)的特征在于PC-8含量(81mg/kg)高于H2-26黑色种子的亲本系(58mg/kg)。两个种群种子中植物甾醇总含量的最大部分是β-谷甾醇,从976到2148mg/kg,紧随其后的是Campastool,从636到1364毫克/千克,和油菜甾醇从375到678mg/kg。总生育酚含量范围为462至595mg/kg(群体HxZ)和460至602mg/kg(群体ZxH)。种子颜色与α-T呈显著正相关(r=0.38,p<0.01),γ-T(r=-0,34,p<0.01)和PC-8含量(r=0.29,p<0.01)。未注意到种子颜色与总生育酚和总植物甾醇含量之间的相关性。
结论:考虑到两个种群的双单倍体之间的遗传变异范围,选择的DH品系可能是进一步育种计划的良好亲本,该计划着重于增加油菜籽油的数量和改善油菜籽油的质量。然而,还将进行进一步的研究,以确定环境对菜籽油中生物活性化合物的影响。亲本DH线的交叉方向:黄色和黑色种子有一定影响,尽管没有统计学意义,关于其种群中平均脂肪的双倍单倍体的多样性,生育色原醇和植物甾醇含量。
BACKGROUND: In the
study, an analysis of tocopherols, plastochomanol-8 and phytosterols was conducted using DH lines obtained from F1 hybrids of reciprocal crosses between yellow- and black-seeded lines.
METHODS: The biological material for the
study consisted of two DH populations of winter oilseed rape obtained from F1 hybrids of reciprocal crosses between two DH lines: yellow- and black-seeded. Seed color was determined using a ColorFlex spectrophotometer. Fat content was determined via pulsed NMR. The levels of tocopherols, and plastochromanol-8 are analyzed using HPLC. Phytosterol contents and composition were determined by the GC method.
RESULTS: The fat content of the black-seeded parental line was 49% and this was higher than that of the yellow-seeded parental line (44%). The fat content of DH line populations ranged from 44 to 51%. Total tocopherol content ranged from 460 to 602 mg/kg and the α-T/γ-T ratio was from 0.66 to 1.09. In parental lines H2-26 and Z-114 the total tocopherol content was 534 and 525 mg/kg, but the α-T/γ-T ratios were 0.81 and 1.21, respectively. The yellow-seeded parental line (Z-114) was characterized by a higher PC-8 content (81 mg/kg) than the H2-26 black-seeded parental line (58 mg/kg). The largest part of the total phytosterol content in seeds of both populations was β-sitosterol from 976 to 2148 mg/kg, followed by campasterol, from 636 to 1364 mg/kg, and brassicasterol from 375 to 678 mg/kg. The total tocopherol content ranged from 462 to 595 mg/kg (population HxZ) and from 460 to 602 mg/kg (population ZxH). Significantly positive correlations were observed between the seed color with α-T (r = 0.38, p < 0.01), γ-T (r = -0,34, p < 0.01) and PC-8 content (r = 0.29, p < 0.01). Correlations between the seed color with total tocopherol and total phytosterol content were not noted.
CONCLUSIONS: Considering the range of genetic variation among doubled haploids of two populations, selected DH lines may be good parents for further breeding programs focused on increasing the amount and improving the quality of oilseed rapeseed oil. However, further studies will also be made to determine the influence of the environment on bioactive compounds in rapeseed oil. Cross direction of parental DH lines: yellow- and black-seeded has some influence, albeit not statistically significant, on the diversity of doubled haploid in their populations with regard to average fat, tocochromanol and phytosterol content.