背景:小麦黑斑病(STB)导致易感小麦品种的产量损失高达50%,并可能降低小麦产量。在这项研究中,在Septoria协会映射小组(SAMP)中寻找成年植物STB抗性的基因组结构,该小组具有181种种质和控制南亚小麦中STB抗性的基因组区域。
结果:2019年至2021年期间的现场实验表明,即BGD52(CHIR7/ANB//CHIR1),BGD54(CHIR7/ANB//CHIR1),IND92(WH1218),IND8(DBW168),IND75(PBW800),表现出很高的抵抗力。遗传分析显示,在所有小麦染色体上都存在21个稳定的数量性状核苷酸(QTNs),与对STB的抗性(Septoriatriticiblotch)相关,除了2D,3A,3D,4A,4D,5D,6B,6D,和7A。这些QTNs主要位于先前鉴定为与STB抗性相关的染色体区域中。在田间评估中发现三个数量性状基因座(QTNs)具有显着的表型效应。这些QTNs是Q.STB.5A.1、Q.STB.5B.1和Q.STB.5B.3。此外,QTNs可能位于染色体1A(Q.STB.1A.1),2A(Q.STB_DH.2A.1,Q.STB.2A.3),2B(Q.STB.2B.4),5A(Q.STB.5A.1,Q.STB.5A.2),7B(Q.STB.7B.2)可能是与抗性相关的新遗传区域。
结论:我们的研究结果表明,亚洲面包小麦作为STB抗性等位基因和新型稳定QTNs的来源对于小麦育种计划的重要性,该计划旨在开发对小麦品种中的TrymoseptoriaTritici的持久和广泛的抗性。
BACKGROUND: Septoria tritici blotch (STB) disease causes yield losses of up to 50 per cent in susceptible
wheat cultivars and can reduce
wheat production. In this
study, genomic architecture for adult-plant STB resistance in a Septoria Association Mapping Panel (SAMP) having 181 accessions and genomic regions governing STB resistance in a South Asian
wheat panel were looked for.
RESULTS: Field experiments during the period from 2019 to 2021 revealed those certain accessions, namely BGD52 (CHIR7/ANB//CHIR1), BGD54 (CHIR7/ANB//CHIR1), IND92 (WH 1218), IND8 (DBW 168), and IND75 (PBW 800), exhibited a high level of resistance. Genetic analysis revealed the presence of 21 stable quantitative trait nucleotides (QTNs) associated with resistance to STB (Septoria tritici blotch) on all wheat chromosomes, except for 2D, 3A, 3D, 4A, 4D, 5D, 6B, 6D, and 7A. These QTNs were predominantly located in chromosome regions previously identified as associated with STB resistance. Three Quantitative Trait Loci (QTNs) were found to have significant phenotypic effects in field evaluations. These QTNs are Q.STB.5A.1, Q.STB.5B.1, and Q.STB.5B.3. Furthermore, it is possible that the QTNs located on chromosomes 1A (Q.STB.1A.1), 2A (Q.STB_DH.2A.1, Q.STB.2A.3), 2B (Q.STB.2B.4), 5A (Q.STB.5A.1, Q.STB.5A.2), and 7B (Q.STB.7B.2) could potentially be new genetic regions associated with resistance.
CONCLUSIONS: Our findings demonstrate the importance of Asian bread wheat as a source of STB resistance alleles and novel stable QTNs for wheat breeding programs aiming to develop long-lasting and wide-ranging resistance to Zymoseptoria tritici in
wheat cultivars.