Effective management of Harmful Algal Blooms (HABs) requires understanding factors influencing their occurrence. This study explores these dynamics in the Pengxi River, a tributary of the Three Gorges Reservoir, focusing on nutrient stratification and algal blooms. We hypothesized that nutrient levels in eutrophic waters with stable stratification correlate with HAB magnitude and that disruption of stratification triggers blooms due to nutrient shifts. A 38-day sampling campaign in Gaoyang Lake (April 16-May 23, 2022) revealed that consistent weather between April 26 and May 16 led to a surface density layer, restricting nutrient transfer and causing a bloom with 173.0 μg L-1 Chl-a on May 1. After a heavy rain on May 18, a peak bloom on May 20, dominated by Ceratium hirundinella, showed 533 μg L-1 Chl-a. There was a significant negative correlation between Cyanobacteria and C. hirundinella biomasses (r = -0.296, P < 0.01), highlighting nutrient availability and physical stability\'s roles in regulating HABs.

Microcystin (MC), a hepatotoxin that is harmful to human health, has frequently increased in freshwaters worldwide due to the increase in toxic cyanobacterial blooms. Despite many studies reported the human exposure to MC through drinking water, the potential transfer of this toxin to human via consumption of vegetables grown on farmlands that are naturally irrigated with contaminated water has not been largely investigated. Therefore, this study investigates the presence of MC in irrigation water and its potential accumulation in commonly consumed vegetables from Egyptian farmlands. The results of toxin analysis revealed that all irrigation water sites contained high MC concentrations (1.3-93.7 μg L-1) along the study period, in association with the abundance of dominant cyanobacteria in these sites. Meanwhile, MCs were detected in most vegetable plants surveyed, with highest levels in potato tubers (1100 μg kg-1 fresh weight, FW) followed by spinach (180 μg kg-1 FW), onion (170 μg g-1 FW), Swiss chard (160 μg kg-1 FW) and fava bean (46 μg kg-1 FW). These MC concentrations in vegetables led to estimated daily intake (EDI) values (0.08-1.13 μg kg bw-1 d-1 for adults and 0.11-1.5 μg kg bw-1 d-1 for children), through food consumption, exceeding the WHO recommended TDI (0.04 μg kg bw-1 d-1) for this toxin. As eutrophic water is widely used for irrigation in many parts of the world, our study suggests that cyanotoxins in irrigation waters and agricultural plants should be regularly monitored to safeguard the general public from inadvertent exposure to harmful toxins via food consumption.

The use of reclaimed water for urban river replenishment has raised concerns regarding its impact on water quality and aquatic ecosystems. This study aims to reveal the improvements seen in an urban river undergoing a practical water eco-remediation after being replenished with reclaimed water. A one-year monitoring of water quality, phytoplankton, and zooplankton was carried out in Dongsha River undergoing eco-remediation in Beijing, China. The results showed that compared to the unrestored river, the concentrations of COD, NH4+-N, TP, and TN decreased by 28.22 ± 7.88 %, 40.24 ± 11.77 %, 44.17 ± 17.29 %, and 28.66 ± 10.39 % in the restoration project area, respectively. The concentration of Chlorophyll-a in the restoration area was maintained below 40 μg/L. During summer, when algal growth is vigorous, the density of Cyanophyta in the unrestored river decreased from 46.84 × 104cells/L to 16.32 × 104cells/L in the restored area, while that of Chlorophyta decreased from 41.61 × 104cells/L to 11.87 × 104cells/L, a reduction of 65.16 % and 71.47 %, respectively. The dominant phytoplankton species were replaced with Bacillariophyta, such as Synedra sp. and Nitzschia sp., indicating that the restoration of aquatic plants reduces the risk of Cyanophyta blooms. Zooplankton species also changed in the restoration area, especially during summer. The density of pollution-tolerant Rotifer and Protozoa decreased by 31.06 % and 27.22 %, while the density of clean water indicating Cladocera increased by 101.19 %. We further calculated the diversity and evenness index of phytoplankton and zooplankton within and outside the restoration area. The results showed that the Shannon-Weaver index for phytoplankton and zooplankton in the restoration area was 2.1 and 1.91, which was higher than those in the river (1.84 and 1.82). This further confirmed that aquatic plant restoration has positive effects. This study can provide a practical reference and theoretical basis for the implementation of water ecological restoration projects in other reclaimed water rivers in China.

Las Tablas de Daimiel National Park (TDNP, Iberian Peninsula) is a semi-arid wetland of international significance for waterfowl and serves as a migratory route for various bird species. However, TDNP presents strong anthropization and fluctuating water levels, making it a highly fragile ecosystem. Water physico-chemical parameters and microbial diversity of the three domains (Bacteria-Archaea- Eukarya) were analysed in Zone A and Zone B of the wetland (a total of eight stations) during spring and summer, aiming to determine how seasonal changes influence the water quality, trophic status and ultimately, the microbial community composition. Additionally, Photosynthetically Active Radiation (PAR) was used to determine the trophic status instead of transparency using Secchi disk, setting the threshold to 20-40 μmol/sm2 for benthic vegetation growth. In spring, both zones of the wetland were considered eutrophic, and physico-chemical parameters as well as microbial diversity were similar to other wetlands, with most abundant bacteria affiliated to Actinobacteriota, Cyanobacteria, Bacteroidota, Gammaproteobacteria and Verrumicrobiota. Methane-related taxa like Methanosarcinales and photosynthetic Chlorophyta were respectively the most representative archaeal and eukaryotic groups. In summer, phytoplankton bloom led by an unclassified Cyanobacteria and mainly alga Hydrodictyon was observed in Zone A, resulting in an increase of turbidity, pH, phosphorus, nitrogen, chlorophyll-a and phycocyanin indicating the change to hypertrophic state. Microbial community composition was geographical and seasonal shaped within the wetland as response to changes in trophic status. Archaeal diversity decreases and methane-related species increase due to sediment disturbance driven by fish activity, wind, and substantial water depth reduction. Zone B in summer suffers less seasonal changes, maintaining the eutrophic state and still detecting macrophyte growth in some stations. This study provides a new understanding of the interdomain microbial adaptation following the ecological evolution of the wetland, which is crucial to knowing these systems that are ecological niches with high environmental value.

Blooms of the red, filamentous cyanobacterium Planktothrix rubescens occur frequently in pre-alpine lakes in Europe, often with concomitant toxic microcystin (MC) production. Trophic transfer of MCs has been observed in bivalves, fish, and zooplankton species, while uptake of MCs into Diptera species could facilitate distribution of MCs into terrestrial food webs and habitats. In this study, we characterized a Planktothrix bloom in summer 2019 in Lake Mindelsee and tracked possible trophic transfer and/or bioaccumulation of MCs via analysis of phytoplankton, zooplankton (Daphnia) and emergent aquatic insects (Chaoborus, Chironomidae and Trichoptera). Using 16 S rRNA gene amplicon sequencing, we found that five sequence variants of Planktothrix spp. were responsible for bloom formation in September and October of 2019, and these MC-producing variants, provisionally identified as P. isothrix and/or P. serta, occurred exclusively in Lake Mindelsee (Germany), while other variants were also detected in nearby Lake Constance. The remaining cyanobacterial community was dominated by Cyanobiaceae species with high species overlap with Lake Constance, suggesting a well-established exchange of cyanobacteria species between the adjacent lakes. With targeted LC-HRMS/MS we identified two MC-congeners, MC-LR and [Asp3]MC-RR with maximum concentrations of 45 ng [Asp3]MC-RR/L in lake water in September. Both MC congeners displayed different predominance patterns, suggesting that two different MC-producing species occurred in a time-dependent manner, whereby [Asp3]MC-RR was clearly associated with the Planktothrix spp. bloom. We demonstrate an exclusive transfer of MC-LR, but not [Asp3]MC-RR, from phytoplankton into zooplankton reaching a 10-fold bioconcentration, yet complete absence of these MC congeners or their conjugates in aquatic insects. The latter demonstrated a limited trophic transfer of MCs from zooplankton to zooplanktivorous insect larvae (e.g., Chaoborus), or direct transfer into other aquatic insects (e.g. Chironomidae and Trichoptera), whether due to avoidance or limited uptake and/or rapid excretion of MCs by higher trophic emergent aquatic insects.

Climate change is anticipated to alter lake ecosystems by affecting water quality, potentially resulting in loss of ecosystem services. Subtropical lakes have high temperatures to begin with and are expected to exhibit higher temperatures all year round which might affect the thermal structure and ecological processes in a different manner than lakes in temperate zones. In this study the ecosystem response of the sub-tropical Lake Kinneret to climate change was explored using lake ecosystem models. Projection reliability was increased by using a weather generator and ensemble modelling, confronting uncertainty of both climate projections and lake models. The study included running two 1D hydrodynamic-biogeochemical models over one thousand realizations of two gradual temperature increase scenarios that span over 49 years. Our predictions show that an increase in air temperature would have subtle effects on stratification properties but may result in considerable changes to biogeochemical processes. Water temperature rise would cause a reduction in dissolved oxygen. Both of these changes would produce elevated phosphate and lowered ammonium concentrations. In turn, these changes are predicted to modify the phytoplankton community, expressed chiefly in increased cyanobacteria blooms at the expense of green phytoplankton and dinoflagellates; these changes may culminate in overall reduction of primary production. Identification of these trends would not be possible without the use of many realizations of climate scenarios. The use of ensemble modelling increased prediction reliability and highlighted elements of uncertainty. Though we use Lake Kinneret, the patterns identified most likely indicate processes that are expected in sub-tropical lakes in general.

Cyanobacteria are aquatic microorganisms of high interest for research due to the production of secondary metabolites, among which the most popular are cyanotoxins, responsible for causing severe poisoning in humans and animals through ingestion or contact with contaminated water bodies. Monitoring the number of cyanobacteria in water and concentrations of secreted cyanotoxins with the aid of sensitive and reliable methods is considered the primary action for evaluating potentially toxic blooms. There is a great diversity of methods to detect and identify these types of micro contaminants in water, differing by the degree of sophistication and information provided. Mass Spectrometry stands out for its accuracy and sensitivity in identifying toxins, making it possible to identify and characterize toxins produced by individual species of cyanobacteria, in low quantities. In this review, we seek to update some information about cyanobacterial peptides, their effects on biological systems, and the importance of the main Mass Spectrometry methods used for detection, extraction, identification and monitoring of cyanotoxins.

The frequency of dogs becoming ill or dying from accidental exposure to cyanotoxins, produced by cyanobacteria, is increasing throughout the United States. In January and February of 2021, two dogs died and five dogs became ill after swimming in Lake Travis, central Texas, USA; one deceased dog (C1) was subjected to pathological testing. Algal materials, sediment samples, zebra mussel viscera, periphyton from shells, as well as fluids and tissues from the digestive tract of C1 were investigated for the following cyanotoxins: anatoxin-a, homoanatoxin-a, dihydroanatoxin-a (dhATX), cylindrospermopsin, saxitoxin, and microcystins. Necropsy results of C1 indicated neurotoxicosis with significant levels of dhATX in the duodenum tissues (10.51 ng/g dry weight (DW)), jejunum tissue (6.076 ng/g DW), and stomach contents (974.88 ng/g DW). Algae collected near the site of C1\'s death contained levels of dhATX, ranging from 13 to 33 µg/g. By comparison, dhATX was detected at much lower concentrations in sediment samples (310.23 ng/g DW) and the periphyton on zebra mussel shells (38.45 ng/g DW). While dhATX was suspected in the deaths of canines from an event in Texas in 2019, this is the first report linking dhATX neurotoxicosis through pathological findings in Texas and potentially in the United States.

Eutrophication of lacustrine environments with frequent cyanobacterial blooms has become a serious problem. Overpopulation and runoff into groundwater and lakes from fertilizers with too much nitrogen and phosphorous have caused most of the problems. Here, we first devised a land use and cover classification system based on the local characteristics of the first-level protected area of Lake Chaohu (FPALC). Lake Chaohu is the fifth largest freshwater lake in China. The land use and cover change (LUCC) products were produced using sub-meter resolution satellite data from 2019 to 2021 in the FPALC. This study presents an evaluation of the effectiveness of the established protected areas. The results show the most impactful type was the reduction in cropland with an area that declined from 7446.4 hm2 to 6433.3 hm2 from 2019 to 2021. Most of the reduced cropland was converted into wetlands, with 460.2 hm2 and 152.0 hm2 of cropland restored to wetlands from 2019 to 2020 and 2020-2021, respectively. The area of cyanobacterial blooms in Lake Chaohu showed a downward trend, and the lacustrine environment was greatly improved after the establishment of the FPALC. These quantified data can inform decision-making related to Lake Chaohu conservation and provide a reference for managing the aquatic environment in other watersheds.

This study was aimed primarily at describing the planktonic assemblages with special attention to invasive and toxin-producing cyanobacterial species in the context of ecological and health threats. The second aim was to analyze the aspect of recreational pressure, which may enhance the cyanobacterial blooms, and, as a consequence, the negative changes and loss of planktonic biodiversity. This study was carried out in recreationally used Lake Sztynorckie throughout the whole growing season of 2020 and included an assessment of the abundance and biomass of phytoplankton (cyanobacteria and algae) in relation to environmental variables. The total biomass was in the range of 28-70 mg L-1, which is typical for strong blooms. The dominant filamentous cyanobacteria were Pseudanabaena limnetica, Limnothrix redekei, Planktolyngbya limnetica, and Planktothrix agarhii, and three invasive nostocalean species Sphaerospermopsis aphanizomenoides, Cuspidothrix issatschenkoi, and Raphidiopsis raciborskii. They can pose a serious threat not only to the ecosystem but also to humans because of the possibility of cyanobacteria producing cyanotoxins, such as microcystins, saxitoxins, anatoxin-a, and cylindrospermopsins, having hepatotoxic, cytotoxic, neurotoxic, and dermatoxic effects. The water quality was assessed as water bodies had bad ecological status (based on phytoplankton), were highly meso-eutrophic (based on zooplankton), and had very low trophic efficiency and low biodiversity.