盐度是影响生长的主要环境因素之一,发展,和大麦的生产力。我们的研究旨在检测在盐度和钾纳米颗粒(n-K)处理下形态和生理性状的自然表型变异。除了了解大麦耐盐性的遗传基础外,植物抗逆性育种的关键方面。因此,在营养阶段对138个大麦品种进行了叶面施用n-K,以增强盐胁迫的复原力。有趣的是,与盐渍土相比,n-K处理下的大麦种质显示出较高的显着增量。基于全基因组关联研究(GWAS)分析,通过钾纳米颗粒的应用,发现了致病等位基因/可靠的基因组区域潜在的盐复原力改善.在染色体2H上,一个高度显著的QTN标记(A:C)位于位置36,665,559bp,与APX相关,AsA,GSH,GS,WGS,和TKW在n-K处理下。在这个区域内,我们的候选基因是HORVU.莫雷克斯.r3.2HG0111480注解为NAC域卵白。等位基因变异检测到携带C等位基因的种质显示出更高的抗氧化剂(APX,AsA,和GSH)和大麦产量性状(GS,WGS,和TKW)比携带A等位基因的种质,建议对携带C等位基因的种质进行积极选择,可用于开发具有改善的盐胁迫恢复力的大麦品种。
Salinity is one of the major environmental factor that can greatly impact the growth, development, and productivity of
barley. Our study aims to detect the natural phenotypic variation of morphological and physiological traits under both salinity and potassium nanoparticles (n-K) treatment. In addition to understanding the genetic basis of salt tolerance in
barley is a critical aspect of plant breeding for stress resilience. Therefore, a foliar application of n-K was applied at the vegetative stage for 138
barley accessions to enhance salt stress resilience. Interestingly,
barley accessions showed high significant increment under n-K treatment compared to saline soil. Based on genome-wide association studies (GWAS) analysis, causative alleles /reliable genomic regions were discovered underlying improved salt resilience through the application of potassium nanoparticles. On chromosome 2H, a highly significant QTN marker (A:C) was located at position 36,665,559 bp which is associated with APX, AsA, GSH, GS, WGS, and TKW under n-K treatment. Inside this region, our candidate gene is HORVU.MOREX.r3.2HG0111480 that annotated as NAC domain protein. Allelic variation detected that the accessions carrying C allele showed higher antioxidants (APX, AsA, and GSH) and barley yield traits (GS, WGS, and TKW) than the accessions carrying A allele, suggesting a positive selection of the accessions carrying C allele that could be used to develop
barley varieties with improved salt stress resilience.