The cap-binding complex modulates ABA-responsive transcript splicing during germination in barley (Hordeum vulgare).

帽结合复合物在大麦 （ 大麦 ） 发芽过程中调节 ABA 响应性转录物剪接。

• 影响指数: 4.996
• 发表时间: 2024 08 7
• 来源期刊: Sci Rep
• DOI: 10.1038/s41598-024-69373-9
• PMID: 39107424
• 文章类型: Journal Article
• 中科院分区: Q1
• 方向: 综合性期刊
• 作者列表: Sybilska E,Collin A,Sadat Haddadi B,Mur LAJ,Beckmann M,Guo W,Simpson CG,Daszkowska-Golec A

关键词: ABA; Alternative splicing; Barley; Cap-binding complex; Embryo; Germination; Transcriptome

Mesh: Hordeum / genetics metabolism growth & development; Germination / genetics; Abscisic Acid / metabolism pharmacology; Gene Expression Regulation, Plant; Plant Proteins / genetics metabolism; RNA Splicing; Mutation; Signal Transduction; Transcriptome; Gene Expression Profiling; RNA Cap-Binding Proteins / metabolism genetics; Seeds / growth & development genetics metabolism

Abstract

To decipher the molecular bases governing seed germination, this study presents the pivotal role of the cap-binding complex (CBC), comprising CBP20 and CBP80, in modulating the inhibitory effects of abscisic acid (ABA) in barley. Using both single and double barley mutants in genes encoding the CBC, we revealed that the double mutant hvcbp20.ab/hvcbp80.b displays ABA insensitivity, in stark contrast to the hypersensitivity observed in single mutants during germination. Our comprehensive transcriptome and metabolome analysis not only identified significant alterations in gene expression and splicing patterns but also underscored the regulatory nexus among CBC, ABA, and brassinosteroid (BR) signaling pathways.

摘要

为了破译控制种子萌发的分子基础，这项研究提出了帽结合复合物(CBC)的关键作用，包括CBP20和CBP80，以调节脱落酸（ABA）在大麦中的抑制作用。在编码CBC的基因中使用单一和双重大麦突变体，我们揭示了双突变体hvcbp20。ab/hvcbp80。B显示ABA不敏感，与发芽过程中单个突变体中观察到的超敏反应形成鲜明对比。我们全面的转录组和代谢组分析不仅发现了基因表达和剪接模式的显著改变，而且强调了CBC之间的调控关系。ABA,和油菜素类固醇(BR)信号通路。