Abstract:
Improving low nitrogen (LN) tolerance in barley (Hordeum vulgare L.) increases global barley yield and quality. In this study, a recombinant inbred line (RIL) population crossed between \"Baudin × CN4079\" was used to conduct field experiments on twenty traits of barley yield, agronomy, and nitrogen(N)-related traits under LN and normal nitrogen (NN) treatments for two years. This study identified seventeen QTL, comprising eight QTL expressed under both LN and NN treatments, eight LN-specific QTL, and one NN-specific QTL. The localized C2 cluster contained QTL controlling yield, agronomic, and N-related traits. Of the four novel QTL, the expression of the N-related QTL Qstna.sau-5H and Qnhi.sau-5H was unaffected by N treatment. Qtgw.sau-2H for thousand-grain weight, Qph.sau-3H for plant height, Qsl.sau-7H for spike length, and Qal.sau-7H for awn length were identified to be the four stable expression QTL. Correlation studies revealed a significant negative correlation between grain N content and harvest index (p < 0.01). These results are essential for barley marker-assisted selection (MAS) breeding.
摘要:
提高大麦(HordeumvulgareL.)的低氮(LN)耐受性可提高全球大麦的产量和质量。在这项研究中,用“Baudin×CN4079”杂交的重组自交系(RIL)群体对大麦产量的20个性状进行田间试验,农学,LN和正常氮(NN)处理两年后,氮(N)相关性状。本研究确定了十七个QTL,包含在LN和NN处理下表达的八个QTL,八个LN特异性QTL,和一个NN特异性QTL。局部C2簇包含控制产量的QTL,农艺,和N相关性状。在四个新颖的QTL中,N相关QTLQstna的表达。sau-5H和Qnhi.sau-5H不受N处理的影响。Qtgw。千粒重的sau-2H,Qph。sau-3H用于植物高度,Qsl.sau-7H为穗长,和Qal。sau-7H的芒长度被鉴定为四个稳定表达的QTL。相关研究表明,籽粒氮含量与收获指数呈极显著负相关(p<0.01)。这些结果对于大麦标记辅助选择(MAS)育种至关重要。