In recent decades, biosynthesis of metal and (or) metal oxide nanoparticles using microbes is accepted as one of the most sustainable, cost-effective, robust, and green processes as it does not encompass the usage of largely hazardous chemicals. Accordingly, numerous simple, inexpensive, and environmentally friendly approaches for the biosynthesis of silver nanoparticles (AgNPs) were reported using microbes avoiding conventional (chemical) methods. This comprehensive review detailed an advance made in recent years in the microbes-mediated biosynthesis of AgNPs and evaluation of their antimicrobial activities covering the literature from 2015-till date. It also aimed at elaborating the possible effect of the different phytochemicals, their concentrations, extraction temperature, extraction solvent, pH, reaction time, reaction temperature, and concentration of precursor on the shape, size, and stability of the synthesized AgNPs. In addition, while trying to understand the antimicrobial activities against targeted pathogenic microbes the probable mechanism of the interaction of produced AgNPs with the cell wall of targeted microbes that led to the cell\'s reputed and death have also been detailed. Lastly, this review detailed the shape and size-dependent antimicrobial activities of the microbes-mediated AgNPs and their enhanced antimicrobial activities by synergetic interaction with known commercially available antibiotic drugs.

Silver nanoparticles are used in laser mass spectrometry to replace organic matrices. Thanks to their unique properties, they enable effective desorption/ionization of samples of various polarities and ionization abilities. This review presents new methods for the synthesis of monoisotopic silver nanoparticles and the use of targets coated with these nanoparticles for qualitative and quantitative analyses of various small-molecule compounds. Additionally, the results of progress in the application of AgNPs for metabolomics analyses were presented.

CONCLUSIONS: The study thoroughly investigates nanosilver production, properties, and interactions, shedding light on its multifaceted applications. It underscores the importance of characterizing nanosilver for predicting its behavior in complex environments. Particularly, it highlights the agricultural and environmental ramifications of nanosilver uptake by plants. Nowadays, silver nanoparticles (AgNPs) are a very adaptable nanomaterial with many uses, particularly in antibacterial treatments and agricultural operations. Clarification of key elements of nanosilver, such as its synthesis and characterization procedures, antibacterial activity, and intricate interactions with plants, particularly those pertaining to uptake and translocation mechanisms, is the aim of this in-depth investigation. Nanosilver synthesis is a multifaceted process that includes a range of methodologies, including chemical, biological, and sustainable approaches that are also environmentally benign. This section provides a critical evaluation of these methods, considering their impacts on repeatability, scalability, and environmental impact. The physicochemical properties of nanosilver were determined by means of characterization procedures. This review highlights the significance of analytical approaches such as spectroscopy, microscopy, and other state-of the-art methods for fully characterizing nanosilver particles. Although grasp of these properties is necessary in order to predict the behavior and potential impacts of nanosilver in complex biological and environmental systems. The second half of this article delves into the intricate interactions that plants have with nanosilver, emphasizing the mechanisms of absorption and translocation. There are significant ramifications for agricultural and environmental problems from the uptake of nanosilver by plants and its subsequent passage through their tissues. In summary, by summarizing the state-of-the-art information in this field, this study offers a comprehensive overview of the production, characterization, antibacterial capabilities, and interactions of nanosilver with plants. This paper contributes to the ongoing conversation in nanotechnology.

The development of new nanocomposites has a significant impact on modern instrumentation and analytical methods for chemical analysis. Due to their unique properties, carbon dots (CDs) and silver nanoparticles (AgNPs), distinguished by their unique physical, electrochemical, and optical properties, have captivated significant attention. Thus, combining AgNPs and CDs may produce Ag/CDs nanocomposites with improved performances than the individual material. This comprehensive review offers an in-depth exploration of the synthesis, formation mechanism, properties, and the recent surge in chemical and biological sensing applications of Ag/CDs with their sensing mechanisms. Detailed insights into synthesis methods to produce Ag/CDs are unveiled, followed by information on their physicochemical and optical properties. The crux of this review lies in its spotlight on the diverse landscape of chemical and biological sensing applications of Ag/CDs, with a particular focus on fluorescence, electrochemical, colorimetric, surface-enhanced Raman spectroscopy, and surface plasmon resonance sensing techniques. The elucidation of sensing mechanisms of the nanocomposites with various target analytes adds depth to the discussion. Finally, this review culminates with a concise summary and a glimpse into future perspectives of Ag/CDs aiming to achieve highly efficient and enduring Ag/CDs for various applications.

The resistance of pathogenic microorganisms to antibiotics is one of the main problems of world health. Of particular concern are multidrug-resistant (MDR) bacteria. Infections caused by these microorganisms affect the appearance of acute or chronic diseases. In this regard, modern technologies, such as nanomaterials (NMs), especially promising nanoparticles (NPs), can possess antimicrobial properties or improve the effectiveness and delivery of known antibiotics. Their diversity and characteristics, combined with surface functionalization, enable multivalent interactions with microbial biomolecules. This article presents an overview of the most current research on replacing antibiotics with NPs, including the prospects and risks involved.

The good antimicrobial properties of silver make it widely used in food, medicine, and environmental applications. However, the release and accumulation of silver-based antimicrobial agents in the environment is increasing with the extensive use of silver-based antimicrobials, and the prevalence of silver-resistant bacteria is increasing. To prevent the emergence of superbugs, it is necessary to exercise rational and strict control over drug use. The mechanism of bacterial resistance to silver has not been fully elucidated, and this article provides a review of the progress of research on the mechanism of bacterial resistance to silver. The results indicate that bacterial resistance to silver can occur through inducing silver particles aggregation and Ag+ reduction, inhibiting silver contact with and entry into cells, efflux of silver particles and Ag+ in cells, and activation of damage repair mechanisms. We propose that the bacterial mechanism of silver resistance involves a combination of interrelated systems. Finally, we discuss how this information can be used to develop the next generation of silver-based antimicrobials and antimicrobial therapies. And some antimicrobial strategies are proposed such as the \"Trojan Horse\" - camouflage, using efflux pump inhibitors to reduce silver efflux, working with \"minesweeper\", immobilization of silver particles.

In recent times, nanoparticles have experienced a significant upsurge in popularity, primarily owing to their minute size and their remarkable ability to modify physical, chemical, and biological properties. This burgeoning interest can be attributed to the expanding array of biomedical applications where nanoparticles find utility. These nanoparticles, typically ranging in size from 10 to 100 nm, exhibit diverse shapes, such as spherical, discoidal, and cylindrical configurations. These variations are not solely influenced by the manufacturing processes but are also intricately linked to interactions with surrounding stabilizing agents and initiators. Nanoparticles can be synthesized through physical or chemical methods, yet the biological approach emerges as the most sustainable and eco-friendly alternative among the three. Among the various nanoparticle types, silver nanoparticles have emerged as the most encountered and widely utilized due to their exceptional properties. What makes the synthesis of silver nanoparticles even more appealing is the application of plant-derived sources as reducing agents. This approach not only proves to be cost-effective but also significantly reduces the synthesis time. Notably, silver nanoparticles produced through plant-mediated processes have garnered considerable attention in recent years due to their notable medicinal capabilities. This comprehensive review primarily delves into the diverse medicinal attributes of silver nanoparticles synthesized using plant-mediated techniques. Encompassing antimicrobial properties, cytotoxicity, wound healing, larvicidal effects, anti-angiogenesis activity, antioxidant potential, and antiplasmodial activity, the paper extensively covers these multifaceted roles. Additionally, an endeavor is made to provide an elucidated summary of the operational mechanisms underlying the pharmacological actions of silver nanoparticles.

Silver nanoparticles (AgNPs) are widely used in various industries, including textiles, electronics, and biomedical fields, due to their unique optical, electronic, and antimicrobial properties. However, the extensive use of AgNPs has raised concerns about their potential ecotoxicity and adverse effects on the environment. AgNPs can enter the environment through different pathways, such as wastewater, surface runoff, and soil application and can interact with living organisms through adsorption, ingestion, and accumulation, causing toxicity and harm. The small size, high surface area-to-volume ratio, and ability to generate reactive oxygen species (ROS) make AgNPs particularly toxic. Various bioremediation strategies, such as phytoremediation, have been proposed to mitigate the toxic effects of AgNPs and minimize their impact on the environment. Further research is needed to improve these strategies and ensure their safety and efficacy in different environmental settings.

In today\'s era, nanoparticles (NPs) have become an integral part of human life, finding extensive applications in various fields of science, pharmacy, medicine, industry, electronics, and communication. The increasing popularity of NP usage worldwide is a testament to their tremendous potential. However, the widespread deployment of NPs unavoidably leads to their release into the environmental matrices, resulting in persistence in ecosystems and bioaccumulation in organisms. Understanding the environmental behavior of NPs poses a significant challenge due to their nanoscale size. Given the current environmental releases of NPs, known negative consequences, and the limited knowledge available for risk management, comprehending the toxicity of NPs in ecosystems is both awaiting and crucial. The present review aims to unravel the potential environmental influences of nano-scaled materials, and provides in-depth inferences of the current knowledge and understanding in this field. The review comprehensively summarizes the sources, fate, transport, toxicity, health risks, and remediation solutions associated with NP pollution in aquatic and soil ecosystems. Furthermore, it addresses the knowledge gaps and outlines further investigation priorities for the sustainable control of NP pollution in these environments. By gaining a holistic understanding of these aspects, we can work toward ensuring the responsible and sustainable use of NPs in today\'s fast-growing world.

Plant pathogens, including bacteria, fungi, and viruses, pose significant challenges to the farming community due to their extensive diversity, the rapidly evolving phenomenon of multi-drug resistance (MDR), and the limited availability of effective control measures. Amid mounting global pressure, particularly from the World Health Organization, to limit the use of antibiotics in agriculture and livestock management, there is increasing consideration of engineered nanomaterials (ENMs) as promising alternatives for antimicrobial applications. Studies focusing on the application of ENMs in the fight against MDR pathogens are receiving increasing attention, driven by significant losses in agriculture and critical knowledge gaps in this crucial field. In this review, we explore the potential contributions of silver nanoparticles (AgNPs) and their nanocomposites in combating plant diseases, within the emerging interdisciplinary arena of nano-phytopathology. AgNPs and their nanocomposites are increasingly acknowledged as promising countermeasures against plant pathogens, owing to their unique physicochemical characteristics and inherent antimicrobial properties. This review explores recent advancements in engineered nanocomposites, highlights their diverse mechanisms for pathogen control, and draws attention to their potential in antibacterial, antifungal, and antiviral applications. In the discussion, we briefly address three crucial dimensions of combating plant pathogens: green synthesis approaches, toxicity-environmental concerns, and factors influencing antimicrobial efficacy. Finally, we outline recent advancements, existing challenges, and prospects in scholarly research to facilitate the integration of nanotechnology across interdisciplinary fields for more effective treatment and prevention of plant diseases.