■骨折被认为是导致严重并发症的医疗紧急情况。
■本研究旨在描述Ag-NPs-FG对兔骨折愈合的加速作用。
■用胡芦巴(FG)还原银NPs(AgNPs),装入淀粉凝胶基质中,并研究了它们的形态,尺寸,和收费。40只成年雄性兔随机组成4组。在每只兔的右胫骨的近端干meta处产生3.5mm直径的骨缺损。第1-4组注射安慰剂生理盐水,AgNPs-FG,普通凝胶,和骨缺损区的FG凝胶,分别。术后8周根据影像学评估愈合情况,骨转换标记,和组织病理学检查。
■获得的AgNPs-FG为淡红色,球形,吸光度为423nm,尺寸为118.0±1.7nm,和-7.8±0.518mV的表面电荷。制备的AgNPs-FG水凝胶清晰,半透明,和同质的。pH值为6.55-6.5±0.2,粘度为4,000和1,875cPs,FG和AgNPs-FG水凝胶的铺展性分别为1.6±0.14和2.0±0.15,分别。与其他治疗组相比,第2组的放射学结合量表显着改善(p<0.05),骨转换标志物显着增加(p<0.05)。组织病理学检查显示,第2组和第4组在术后第28天形成成熟骨。
■载有AgNPs-FG水凝胶的胶体纳米制剂可能是加速兔胫骨骨愈合过程的有前途的制剂。
UNASSIGNED: A fracture is considered a medical emergency leading to considerable complications.
UNASSIGNED: This study aimed to describe the accelerating action of Ag-NPs-FG on fracture healing in rabbits.
UNASSIGNED: Silver NPs (AgNPs) were reduced with fenugreek (FG), loaded into a starch gel base, and investigated for their morphology, size, and charge. Four equal groups were randomly formed of 40 adult male rabbits. A 3.5 mm diameter bone defect was created at the proximal metaphysis of the right tibia in each rabbit. Groups 1-4 were injected with placebo saline, AgNPs-FG, plain gel, and FG-gel at the bone defect zone, respectively. The healing was assessed for 8 weeks postoperatively based on the radiographic, bone turnover markers, and histopathological examinations.
UNASSIGNED: The AgNPs-FG was obtained as a faint reddish color, spherical in shape, with an absorbance of 423 nm, a size of 118.0 ± 1.7 nm, and a surface charge of -7.8 ± 0.518 mV. The prepared AgNPs-FG hydrogel was clear, translucent, and homogenous. The pH values were 6.55-6.5 ± 0.2, the viscosity of 4,000 and 1,875 cPs, and spreadability of 1.6 ± 0.14 and 2.0 ± 0.15 for both FG and AgNPs-FG hydrogel, respectively. The radiographic union scale was significantly (p < 0.05) improved in group 2 with a significant (p < 0.05) increase in bone turnover markers was found in comparison to other treated groups. Histopathological examination revealed the formation of mature bone on the 28th postoperative day in groups 2 and 4.
UNASSIGNED: Colloidal nano-formulation of AgNPs-FG loaded hydrogel could be a promising formulation to accelerate rabbits\' tibial bone healing process.