这项研究描述了氧化锌(ZnO)纳米颗粒和微米级颗粒的毒性行为的体外比较研究。该研究旨在通过表征不同介质中的颗粒来了解颗粒大小对ZnO毒性的影响。包括细胞培养基,人血浆,和蛋白质溶液(牛血清白蛋白和纤维蛋白原)。在研究中使用多种方法对颗粒及其与蛋白质的相互作用进行了表征,包括原子力显微镜(AFM),透射电子显微镜(TEM),和动态光散射(DLS)。溶血活性,凝血时间,和细胞活力测定用于评估ZnO毒性。结果强调了ZnONPs与生物系统之间的复杂相互作用,包括它们的聚集行为,溶血活性,蛋白质电晕形成,凝血效应,和细胞毒性。此外,研究表明,ZnO纳米颗粒的毒性并不比微米级颗粒高,和50纳米粒子的结果是,总的来说,毒性最小。此外,研究发现,在低浓度下,未观察到急性毒性。总的来说,这项研究为ZnO颗粒的毒性行为提供了重要的见解,并强调纳米尺寸与毒性之间没有直接关系。
This
study describes a comparative in vitro
study of the toxicity behavior of zinc oxide (ZnO) nanoparticles and micro-sized particles. The
study aimed to understand the impact of particle size on ZnO toxicity by characterizing the particles in different media, including cell culture media, human plasma, and protein solutions (bovine serum albumin and fibrinogen). The particles and their interactions with proteins were characterized in the
study using a variety of methods, including atomic force microscopy (AFM), transmission electron microscopy (TEM), and dynamic light scattering (DLS). Hemolytic activity, coagulation time, and cell viability assays were used to assess ZnO toxicity. The results highlight the complex interactions between ZnO NPs and biological systems, including their aggregation behavior, hemolytic activity, protein corona formation, coagulation effects, and cytotoxicity. Additionally, the
study indicates that ZnO nanoparticles are not more toxic than micro-sized particles, and the 50 nm particle results were, in general, the least toxic. Furthermore, the
study found that, at low concentrations, no acute toxicity was observed. Overall, this study provides important insights into the toxicity behavior of ZnO particles and highlights that no direct relationship between nanometer size and toxicity can be directly attributed.