Abstract：
Biomedical applications of nanoparticles require a fundamental understanding of their interactions and behavior with biological interfaces. Protein corona formation can alter the morphology and properties of nanomaterials, and knowledge of the interfacial behavior of the complexes, using in situ analytical techniques, will impact the development of nanocarriers to maximize uptake and permeability at cellular interfaces. In this study we evaluate the interactions of acrylamide-based nanogels, with neutral, positive, and negative charges, with serum-abundant proteins albumin, fibrinogen, and immunoglobulin G. The formation of a protein corona complex between positively charged nanoparticles and albumin is characterized by dynamic light scattering, circular dichroism, and surface tensiometry; we use neutron reflectometry to resolve the complex structure at the air/water interface and demonstrate the effect of increased protein concentration on the interface. Surface tensiometry data suggest that the structure of the proteins can impact the interfacial properties of the complex formed. These results contribute to the understanding of the factors that influence the bio-nano interface, which will help to design nanomaterials with improved properties for applications in drug delivery.
摘要：
纳米粒子的生物医学应用需要对它们与生物界面的相互作用和行为有基本的了解。蛋白质电晕的形成可以改变纳米材料的形态和性质,以及复合物界面行为的知识，使用原位分析技术，将影响纳米载体的发展，以最大限度地提高细胞界面的吸收和渗透性。在这项研究中，我们评估了丙烯酰胺基纳米凝胶的相互作用，中立的,积极的,和负电荷，血清丰富的蛋白质白蛋白，纤维蛋白原，和免疫球蛋白G。带正电荷的纳米颗粒和白蛋白之间的蛋白质电晕复合物的形成通过动态光散射表征，圆二色性,和表面张力法；我们使用中子反射法来解析空气/水界面处的复杂结构，并证明蛋白质浓度增加对界面的影响。表面张力测定数据表明蛋白质的结构可以影响所形成的复合物的界面性质。这些结果有助于理解影响生物纳米界面的因素，这将有助于设计具有改进性能的纳米材料，用于药物输送。
