背景:指示剂氨基酸氧化(IAAO)方法已被接受为在自由生活条件下评估习惯性蛋白质需求的方法。
目的:本范围综述报告了使用IAAO方法评估人类蛋白质需求的文献。
方法:三个数据库(PubMed/Medline,WebofScience,和ProQuest)进行了系统搜索,以确定使用英文发表的IAAO方法评估蛋白质需求的研究,直到2023年6月5日。我们使用先前制定的标准评估研究质量。我们提取了研究设计的特征和蛋白质要求的结果。两名审稿人独立进行了审查和质量评估;他们之间的任何分歧都通过所有团队成员的共识或同意来解决。
结果:我们提取了16篇针对儿童的文章,年轻人(包括孕妇,阻力训练运动员,耐力训练运动员,和团队运动),和老年人。对于质量评估,14项研究被评估为“强”,但剩下的两个是“适度的”。这些研究仅在三个国家进行,并非针对所有性别或生命阶段。估计每个生命阶段的平均蛋白质需求范围为1.3g/kg体重/天的儿童,0.87至2.1(一般年轻人为0.87-0.93,1.22-1.52孕妇,1.49-2.0用于抵抗训练的运动员,1.65-2.1对于耐力运动员,1.2-1.41对于团队运动运动员)g/kg体重/天对于年轻人,和0.85至0.96g/kg体重/天的老年人。
结论:在14项研究中,每种性别的蛋白质需求均高于目前的参考值,与蛋白质需求相关的生命阶段和身体活动。在未来,包括性别在内的各种人群的蛋白质需求,生命阶段可以在全球范围内使用IAAO方法进行评估。
The indicator amino acid oxidation (IAAO) method has been accepted as an approach to evaluate habitual protein requirements under free-living conditions.
This scoping
review reports on literature that evaluated protein requirements in humans using the IAAO methods.
Three databases (PubMed/Medline, Web of Science, and ProQuest) were systematically searched to identify studies that evaluated protein requirements using the IAAO method published in English until 5 June, 2023. We evaluated the study quality using previously developed criteria. We extracted the characteristics of the study design and the results of protein requirements. Two reviewers conducted both reviews and quality assessments independently; any differences among them were resolved by consensus or agreement of all team members.
We extracted 16 articles targeting children, young adults (including pregnant women, resistance training athletes, endurance-training athletes, and team sports), and older adults. In quality assessment, 14 studies were evaluated \"strong,\" but the remaining 2 were \"moderate.\" These studies were conducted in only 3 countries and did not include all sexes or life stages. The range of the estimated average protein requirements of each life stage was 1.30 g/kg body weight/d for children, 0.87 to 2.10 (0.87-0.93 for general young adults, 1.22-1.52 for pregnant women, 1.49-2.00 for resistance-trained athletes, 1.65-2.10 for endurance athletes, and 1.20-1.41 for team sports athletes) g/kg body weight/d for young adults, and 0.85 to 0.96 g/kg body weight/d for older adults.
Protein requirements in 14 studies were higher than the current reference for each sex, life stage, and physical activity that are related to protein requirements. In the future, protein requirements of various populations including sex and life stage could be assessed using the IAAO methods worldwide.