对羟基苯甲酸酯由于其抗真菌和抗微生物作用而被用作防腐剂。由于在许多产品中过度使用,它们正在成为水生污染物。这项研究的目的是确定对羟基苯甲酸乙酯(C9H10O3)对血液生化,组织学,氧化,和抗氧化酶活性和非酶活性;该研究还评估了对羟基苯甲酸乙酯在RohuLabeorohita中引起遗传毒性的潜力。每组放置15条鱼,平均体重为35.45±1.34g,并暴露于对羟基苯甲酸乙酯21天。三种不同浓度的对羟基苯甲酸乙酯,即,T1(2000μg/L),T2(4000μg/L),与对照T0(0.00μg/L)相比,鱼暴露的T3(6000μg/L)。血液用于血液生化和彗星测定。Gills,肾脏,切除肝脏进行组织学改变。结果表明,所有血液生化参数如红细胞显着上升,WBC,PLT计数,血糖,白蛋白,球蛋白,和胆固醇。天冬氨酸转氨酶(AST)和丙氨酸转氨酶(ALT)水平的增加导致肝细胞损伤。肝脏的组织学改变,发现了鱼的g和肾脏。对羟基苯甲酸乙酯通过抑制抗氧化酶活性如SOD,GSH,CAT和POD。根据彗星试验,在血细胞中也观察到DNA损伤,导致遗传毒性。本研究的结果表明,对羟基苯甲酸乙酯诱导血液生化改变,组织损伤,氧化应激,和遗传毒性。
Parabens are being used as preservatives due to their antifungal and antimicrobial effects. They are emerging as aquatic pollutants due to their excessive use in many products. The purpose of this
study was to determine the toxic effect of ethyl paraben (C9H10O3) on the hematobiochemical, histological, oxidative, and anti-oxidant enzymatic and non-enzymatic activity; the
study also evaluates the potential of ethyl paraben to cause genotoxicity in Rohu Labeo rohita. A number of 15 fish with an average weight of 35.45±1.34g were placed in each group and exposed to ethyl paraben for 21 days. Three different concentrations of ethyl paraben, i.e., T1 (2000μg/L), T2 (4000 μg/L), andT3 (6000 μg/L) on which fish were exposed as compared to the control T0 (0.00 μg/L). Blood was used for hematobiochemical and comet assay.
Gills, kidneys, and liver were removed for histological alterations. The results showed a significant rise in all hemato-biochemical parameters such as RBCs, WBCs, PLT count, blood sugar, albumin, globulin, and cholesterol. An increase in aspartate aminotransferase (AST) and alanine transaminase (ALT) levels directed the hepatocytic damage. Histological alterations in the liver,
gills and kidneys of fish were found. Ethylparaben induces oxidative stress by suppressing antioxidant enzyme activity such as SOD, GSH, CAT and POD. Based on the comet assay, DNA damage was also observed in blood cells, resulting in genotoxicity. Findings from the present
study indicate that ethyl paraben induces hemato-biochemical alterations, tissue damage, oxidative stress, and genotoxicity.