Abstract：
The aim of this study was to investigate microplastic (MP) exposure by Nile tilapia (Oreochromis niloticus) in laboratory conditions. A total of 150 tilapia fishes were equally distributed randomly in 15 different glass tanks with five experimental conditions. Observed results depicted that the presence of MPs in different organs was mainly accumulated from the fish feed rather than externally added MPs in the culture tanks. It was also revealed that the gastrointestinal tract (GIT) was found to be the most susceptible to MPs accumulation followed by gills and muscles in order. However, muscle contained the least size of MPs followed by GITs and gills. A statistical test showed significant correlations among the average length and weight of fish with MP exposure. A filamentous shape was found to be dominant in both GITs and gills while fragment shape was dominant in muscles. FTIR results revealed a total of 12 different polymers in the fish of which two polymers (polyvinyl alcohol and ethylene vinyl acetate) were not detected in the feed-only tanks. Polypropylene (PP) and polyethylene terephthalate (PET) were found to be dominant polymers in all the experimental GIT, gills, and muscle organs. FESEM results indicated the presence of different textures including cracks, edges, flakes, scratches, grooves, and adhering particles. EDX results exhibited the presence of Na, Si, K, Ni, Cu, Zn, As, and Cd in the analyzed samples that may pose additional health risks. Thus, this study could act as baseline data for laboratory-based studies of aquaculture species in future research.
摘要：
这项研究的目的是在实验室条件下调查尼罗罗非鱼（Oreochromisniloticus）的微塑料（MP）暴露。在5个实验条件下，将150条罗非鱼随机平均分布在15个不同的玻璃罐中。观察到的结果表明，不同器官中MP的存在主要是从鱼饲料中积累的，而不是从培养槽中外部添加的MP。还发现，胃肠道（GIT）最容易受到MP积累的影响，其次是g和肌肉。然而,肌肉包含最小大小的MP，其次是GIT和g。统计测试表明，鱼的平均长度和重量与MP暴露之间存在显着相关性。发现丝状形状在GIT和g中均占主导地位，而碎片形状在肌肉中占主导地位。FTIR结果表明，鱼中总共有12种不同的聚合物，其中两种聚合物（聚乙烯醇和乙烯乙酸乙烯酯）在仅饲料罐中未检测到。聚丙烯（PP）和聚对苯二甲酸乙二醇酯（PET）被发现是所有实验GIT中的主要聚合物，ill,和肌肉器官。FESEM结果表明存在不同的纹理，包括裂纹，边缘,薄片,划痕,凹槽,和粘附的颗粒。EDX结果显示Na的存在，Si,K,Ni,Cu,Zn,As,分析样品中的Cd和Cd可能会带来额外的健康风险。因此，这项研究可以作为未来研究中水产养殖物种实验室研究的基线数据.
