在过去的10年里,基因治疗领域的纳米粒子取得了显著进展;然而,低递送效率和其他原因阻碍了纳米载体的临床转化。因此,需要总结该领域的热点和趋势,以促进进一步的研究发展。在这项研究中,从2011年到2021年,CiteSpace分析了1,221条关于纳米颗粒靶向递送系统的WebofScience索引手稿的完整记录和引用参考文献,VOSviewer,和MapEquation。在这些软件中,关键词共现网络,冲积图,共同引文网络,并进行了结构变异分析,以强调科学界对核酸靶向递送的纳米医学的关注。关键词如转染效率,肿瘤细胞,膜抗原,和siRNA递送在来自VOSviewer的密度图中突出显示。此外,构建了冲积层流程图来检测概念的变化.在共同引文网络中,簇1(外泌体)和簇17(基因组编辑)是新的研究领域,并且在结构变异分析中揭示了修饰纳米粒子的努力。适体和SELEX(通过指数富集的配体的系统进化)代表了靶向递送中的有用系统。这些结果表明纳米载体的转染效率需要持续改进。随着几种核酸药物的批准,纳米颗粒载体的新内容是引入基因编辑技术,特别是CRISPR/Cas9(成簇规则间隔的短回文重复/CRISPR相关蛋白9)。此外,外泌体具有作为靶向纳米颗粒的巨大潜力。通过绘制纳米医学在核酸靶向递送中的知识领域,本研究分析了该领域近10年的知识结构,强调对纳米粒子的经典修改,并为对该领域感兴趣的研究人员和决策者估计未来的趋势。
Nanoparticles for the gene therapy field have seen remarkable progress over the last 10 years; however, low delivery efficiency and other reasons impede the clinical translation of nanocarriers. Therefore, a summary of hotspots and trends in this field is needed to promote further research development. In this research, from 2011 to 2021, 1,221 full records and cited references of Web of Science-indexed manuscripts regarding nanoparticle-targeted delivery systems have been analyzed by CiteSpace, VOSviewer, and MapEquation. In these software, keywords co-occurrence networks, alluvial diagram, co-citation networks, and structural variation analysis were carried out to emphasize the scientific community\'s focus on nanomedicine of targeted delivering of nucleic acids. Keywords such as transfection efficiency, tumor cell, membrane antigen, and siRNA delivery were highlighted in the density map from VOSviewer. In addition, an alluvial flow diagram was constructed to detect changes in concepts. In the co-citation network, cluster 1 (exosomes) and cluster 17 (genome editing) were new research fields, and the efforts in modifying nanoparticles were revealed in the structural variation analysis. Aptamer and SELEX (systematic evolution of ligands by exponential enrichment) represented a helpful system in targeted delivery. These results indicated that the transfection efficiency of nanocarriers required continuous improvements. With the approval of several nucleic acid drugs, a new content of nanoparticle carriers is to introduce gene-editing technology, especially CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9). In addition, exosomes have great potential as targeted nanoparticles. By mapping the knowledge domains of nanomedicine in targeted delivering of nucleic acids, this
study analyzed the intellectual structure of this domain in the recent 10 years, highlighting classical modifications on nanoparticles and estimating future trends for researchers and decision-makers interested in this field.