背景:山药,一种山药,是一种药用和食用植物,在中国用于健脾和胃。然而,缺乏关于其抗胃损伤活性的现代药理学研究。
目的:本研究旨在研究山药水提物(CYW)的植物化学成分,并评价其对乙醇引起的胃损伤的内外保护作用。
方法:使用HPLC-QTOF-MS/MS结合GNPS分子网络和网络药理学鉴定CYW的活性成分。在RAW264.7/GES-1细胞共培养系统中进行体外研究。体内研究,小鼠用CYW(0.31、0.63和3.14g/kgBW,口服)14天,然后单剂量口服乙醇(10mL/kgBW)诱导胃损伤。生化,使用商业试剂盒分析炎症和氧化应激标志物。组织病理学用于评估胃损伤的程度。使用RT-qPCR和蛋白质印迹法研究基因和蛋白质表达,分别。
结果:CYW显著恢复SOD水平,GPx和CAT,并降低了MDA含量。进一步分析表明,CYW通过降低p-NF-κB和p-IκB-α的表达水平,抑制IL-6、TNF-α的生成,从而显著减轻胃黏膜氧化应激,和IL-1β。同时,该部分显着上调Bcl-2,下调Bax并增加生长因子的分泌,从而阻止胃粘膜细胞。此外,HPLC-QTOF-MS/MS联用,GNPS分子网络分析,和网络药理学证明了亚油酸,3-乙酰基-11-酮-β-乳香酸,腺苷,氨基己酸,酪胺,DL-色氨酸,环亮氨酸,乳果糖,Melibose,α-β-海藻糖,和蔗糖将是CYW抗乙醇诱导的胃损伤的主要活性化合物。
结论:本研究表明CYW是抗氧化和抗炎生物活性化合物的潜在丰富来源。它通过抑制炎症表现出对乙醇引起的胃损伤的功效,氧化应激,以及胃中的细胞凋亡。当前工作的结果表明,山药可用作生产新药和功能性食品的一种自然资源,以预防和/或改善乙醇引起的胃损伤。
BACKGROUND: Dioscoreae Rhizoma, a kind of Chinese yam, is a medicinal and edible plant used in China for strengthening the spleen and stomach. However, there is a lack of modern pharmacology studies regarding its anti-gastric injury activity.
OBJECTIVE: This
study aimed to investigate the phytochemical composition of Chinese yam aqueous extract (CYW) and evaluate its gastroprotective effects against ethanol-induced gastric injury in vitro and in vivo.
METHODS: The active components of CYW were identified using HPLC-QTOF-MS/MS in combination with the GNPS molecular networking and network pharmacology. In vitro studies were performed in the RAW264.7/GES-1 cell coculture system. In vivo
study, mice were treated with CYW (0.31, 0.63, and 3.14 g/kg BW, orally) for 14 days, followed by a single oral dose of ethanol (10 mL/kg BW) to induce gastric injury. The biochemical, inflammation and oxidative stress markers were analyzed using commercial kits. Histopathology was used to assess the degree of gastric injury. Gene and protein expressions were studied using RT-qPCR and western blotting, respectively.
RESULTS: CYW significantly restored the levels of SOD, GPx and CAT, and reduced the MDA content. Further analyses showed that CYW significantly alleviated the gastric oxidative stress by inhibiting the inflammation via decreasing p-NF-κB and p-IκB-α expression levels and inhibiting the generation of IL-6, TNF-α, and IL-1β. At the same time, the fraction remarkably upregulated Bcl-2, downregulated Bax and increased growth factor secretion, thereby prevented gastric mucous cell. Besides, The combination of HPLC-QTOF-MS/MS, GNPS molecular networking analysis, and network pharmacology demonstrated that linoleic acid, 3-acetyl-11-keto-beta-boswellic acid, adenosine, aminocaproic acid, tyramine, DL-tryptophan, cycloleucine, lactulose, melibiose, alpha-beta-trehalose, and sucrose would be the main active compounds of CYW against ethanol-induced gastric injury.
CONCLUSIONS: This
study showed that CYW is potentially rich source of anti-oxidant and anti-inflammatory bioactive compounds. It showed efficacy against ethanol-induced gastric injury by inhibiting inflammation, oxidative stress, and apoptosis in the stomach. The results of the current work indicate that Dioscoreae Rhizoma could be utilized as a type of natural resource for production of new medicine and functional foods to prevent and/or ameliorate ethanol-induced gastric injury.