目的:一些消毒剂和消毒剂用于牙科,并且有许多商业解决方案可用。尽管如此,因为每种清洁溶液都有自己的指示和限制,处理所有类型的牙科设备没有一刀切的方法。功能水,如电解次氯酸盐微泡水,有效地消除和消毒生物膜。该研究的目的是评估臭氧水是否可用于对已被微生物污染的牙科单位水管(DUWP)进行灭菌和消毒。包括革兰氏阳性和革兰氏阴性杆菌和球菌。
方法:分为三个不同的组:A组-臭氧化水(Cantoosh);B组-1%碘碘;C组:常规蒸馏水。A组为试验组,B组为对照组,C组为阳性对照组。根据分配的组分类,用适当的灭菌剂替换水灭菌系统。吹扫2分钟,这样完整的DUWP就充满了水消毒系统。收集和分析样本,在24小时后进行2分钟的吹扫,7天和21天,在3个出口(OL)点:牙科托盘(OL1)处的3向注射器,杯填充器(OL2),和辅助区的3路注射器(OL3)。重复测量ANOVA用于测试对照组和实验组的集落形成单位之间的统计学显著性(p<0.05)。
结果:在牙科托盘(OL1)上,杯子填充物的计数高于3向注射器的计数(对数刻度为6.40和8.05,分别)。在24小时后采集的样本与A组之间的21天之间的CFU之间也观察到了统计学上的显着差异。B和C.
结论:研究结果表明,将DUWP管系统长时间暴露于臭氧化水中会大大降低粘附在其表面的微生物数量。
OBJECTIVE: A number of disinfectants and sanitisers are used in dentistry, and there are numerous commercial solutions available. Nonetheless, because each cleaning solution has its own set of indications and limits, there is no one-size-fits-all approach for processing all types of dental equipment. Functional water, such as electrolysed hypochlorite microbubbled water, efficiently eliminates and sterilises biofilms. The objective of the
study was to evaluate whether ozonated water could be used to sterilise and disinfect dental-unit water pipelines (DUWP) that had been contaminated with micro-organisms, including Gram-positive and Gram-negative bacilli and cocci.
METHODS: Three different groups were formed: group A - ozonated water (Cantoosh); group B - 1% povidine iodine; and group C: conventional distilled water. Group A was the test group, group B the control group, and group C was the positive control group. The water sterilising system was replaced with the appropriate sterilising agent as per the allocated group classification, with 2 min of purging, so that the complete DUWP was filled with the water sterilising system. Samples were collected and analysed, along with a 2-min purge after 24 h, 7 days and 21 days, at the 3 outlet (OL) points: the 3-way syringe at the dental tray(OL1), the cup filler (OL2), and the 3-way syringe of the assistant zone (OL3). Repeated measures ANOVA was used to test for statistical significance between colony-forming units of control and experimental groups (p < 0.05).
RESULTS: The cup filler yielded higher counts than did the 3-way syringe at the dental tray (OL1) (6.40 and 8.05 on the log scale, respectively). A statistically significant difference in the CFUs was also observed between samples taken after 24 h vs 21 days between groups A, B and C.
CONCLUSIONS: The findings showed that exposing DUWP tube systems to ozonated water for an extended length of time drastically lowered the number of microorganisms adhering to their surfaces.