Mesh : India Dental Clinics Equipment Contamination Water Microbiology Dental Equipment / microbiology Humans Legionella / isolation & purification Pseudomonas aeruginosa / isolation & purification Acinetobacter / isolation & purification In Vitro Techniques

来  源:   DOI:10.4103/ijdr.ijdr_463_22

Abstract:
BACKGROUND: Dental Unit Water Line (DUWL) deliver water to different handpieces in a dental unit. The water in DUWL circulates in a closed system, where it is taken from a container. The quality of dental water is of considerable importance since patients and dental staff are regularly exposed to water and aerosols generated from dental equipment. Output water from DUWLs may be a potential source of infection for both dental health care personnel and patients.
OBJECTIVE: To assess the microbial contamination in the DUWL among dental clinics in Chennai.
METHODS: An in vitro study was conducted on 60 water samples from 20 dental clinics in Chennai in December 2019. Water samples were collected from three different sources of the Dental unit according to ADA guidelines. The collected samples were assessed for the presence of Aspergillus, Acinetobacter, Pseudomonas aeruginosa, and Legionella by agar plate method. The data were analysed using SPSS software version 20.
RESULTS: Legionella was the most prevalent microorganism with 70% prevalence in a three-way syringe and 50% in scaler and airotor, followed by Pseudomonas aeruginosa and Acinetobacter with 10% prevalence in scaler and airotor and Aspergillus with a prevalence of 10% in the three-way syringe.
CONCLUSIONS: Most of the dental units were contaminated with Aspergillus, Legionella, Pseudomonas aeruginosa and Acinetobacter which pose a serious threat to the patients as well as the dentists.
摘要:
背景:牙科单元水线(DUWL)将水输送到牙科单元中的不同手持件。DUWL中的水在封闭系统中循环,它是从容器中取出的。牙科水的质量是相当重要的,因为患者和牙科工作人员经常暴露于由牙科设备产生的水和气溶胶。来自DUWL的输出水可能是牙科保健人员和患者的潜在感染源。
目的:评估钦奈牙科诊所DUWL中的微生物污染。
方法:于2019年12月对来自钦奈20个牙科诊所的60个水样进行了体外研究。根据ADA指南从牙科单元的三个不同来源收集水样。评估收集的样品是否存在曲霉,不动杆菌,铜绿假单胞菌,和军团菌采用琼脂平板法。使用SPSS软件版本20对数据进行分析。
结果:军团菌是最普遍的微生物,在三通注射器中患病率为70%,在洁牙器和旋转器中患病率为50%,其次是铜绿假单胞菌和不动杆菌,在洁牙器和转体中的患病率为10%,在三通注射器中的患病率为10%。
结论:大多数牙科单元被曲霉污染,军团菌,铜绿假单胞菌和不动杆菌对患者和牙医构成严重威胁。
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