METHODS: Chlorine dioxide disinfectants at concentrations of 5, 20, and 80 mg/L were used to treat biofilms of S. aureus and E. coli cultured in DUWLs. The disinfection effectiveness was assessed through bacterial counts and culturing. Simultaneously, human skin fibroblast cells were treated with the disinfectant to observe changes in cell morphology and cytotoxicity. Additionally, the study included corrosion tests on various metals (carbon steel, brass, stainless steel, aluminum, etc.).
RESULTS: Experimental results showed that chlorine dioxide disinfectants at concentrations of 20 mg/L and 80 mg/L significantly reduced the bacterial count of S. aureus and E. coli, indicating effective disinfection. In terms of cytotoxicity, higher concentrations were more harmful to cellular safety, but even at 80 mg/L, the cytotoxicity of chlorine dioxide remained within controllable limits. Corrosion tests revealed that chlorine dioxide disinfectants had a certain corrosive effect on carbon steel and brass, and the degree of corrosion increased with the concentration of the disinfectant.
CONCLUSIONS: After thorough research, we recommend using chlorine dioxide disinfectant at a concentration of 20 mg/L for significantly reducing bacterial biofilms in dental unit waterlines (DUWLs). This concentration also ensures satisfactory cell safety and metal corrosion resistance.
方法:使用浓度为5、20和80mg/L的二氧化氯消毒剂处理在DUWL中培养的金黄色葡萄球菌和大肠杆菌的生物膜。通过细菌计数和培养评估消毒效果。同时,用消毒剂处理人皮肤成纤维细胞,观察细胞形态和细胞毒性的变化。此外,这项研究包括对各种金属(碳钢,黄铜,不锈钢,铝,等。).
结果:实验结果表明,浓度为20mg/L和80mg/L的二氧化氯消毒剂显着降低了金黄色葡萄球菌和大肠杆菌的细菌数量,表明有效的消毒。在细胞毒性方面,更高的浓度对细胞安全更有害,但即使是80毫克/升,二氧化氯的细胞毒性保持在可控范围内。腐蚀试验表明,二氧化氯消毒剂对碳钢和黄铜有一定的腐蚀作用,腐蚀程度随消毒剂浓度的增加而增加。
结论:经过深入研究,我们建议使用浓度为20mg/L的二氧化氯消毒剂来显着减少牙科单位水线(DUWL)中的细菌生物膜。该浓度还确保了令人满意的电池安全性和耐金属腐蚀性。