背景:线粒体,对于通过氧化磷酸化(OXPHOS)产生细胞能量至关重要,整合mt-DNA和核编码基因。这种合作延伸到线粒体翻译机器,涉及关键的mtDNA编码的RNA:22个tRNA(mt-tRNA)作为衔接子,两个rRNA(mt-rRNA)用于核糖体组装,使线粒体编码的mRNA翻译。线粒体基因表达的中断可以强烈影响能量产生和整体动物健康。我们的研究调查了水牛中mt-tRNA和mt-rRNA的组织特异性表达模式。
方法:为了研究mt-tRNAs和mt-rRNAs在不同组织中的表达模式,并更好地了解组织特异性变异,RNA-seq在各种组织上进行,比如肾脏,心,大脑,和卵巢,来自青春期后的雌性水牛。随后,我们确定了在各种组织比较中差异表达的转录本.
结果:这些发现揭示了不同组织中特定mt-tRNA和mt-rRNA基因的不同表达模式,在特定组织环境中,一些表现出显着的上调,另一些表现出明显的下调。这些确定的变化反映了组织特异性的生理作用,强调它们在满足每个组织独特的能量需求方面的重要性。值得注意的是,大脑表现出最高的mtDNA拷贝数和我们早期发现的丰富的线粒体mRNA,可能与大脑中mt-tRNAs的显著上调有关。这表明mtDNA复制与mtDNA基因表达的调节之间存在合理的关联。
结论:总体而言,我们的研究揭示了线粒体编码的非编码RNA在水牛中的组织特异性表达。随着我们的前进,我们对组织特异性线粒体蛋白质组学和microRNA研究的进一步研究旨在阐明线粒体内的复杂机制,有助于组织特异性线粒体属性。这项研究有望阐明线粒体在动物健康和疾病中的关键作用。
BACKGROUND: Mitochondria, essential for cellular energy production through oxidative phosphorylation (OXPHOS), integrate mt-DNA and nuclear-encoded genes. This cooperation extends to the mitochondrial translation machinery, involving crucial mtDNA-encoded RNAs: 22 tRNAs (mt-tRNAs) as adapters and two rRNAs (mt-rRNAs) for ribosomal assembly, enabling mitochondrial-encoded mRNA translation. Disruptions in mitochondrial gene expression can strongly impact energy generation and overall animal health. Our study investigates the tissue-specific expression patterns of mt-tRNAs and mt-rRNAs in buffalo.
METHODS: To investigate the expression patterns of mt-tRNAs and mt-rRNAs in different tissues and gain a better understanding of tissue-specific variations, RNA-seq was performed on various tissues, such as the kidney, heart, brain, and ovary, from post-pubertal female
buffaloes. Subsequently, we identified transcripts that were differentially expressed in various tissue comparisons.
RESULTS: The findings reveal distinct expression patterns among specific mt-tRNA and mt-rRNA genes across various tissues, with some exhibiting significant upregulation and others demonstrating marked downregulation in specific tissue contexts. These identified variations reflect tissue-specific physiological roles, underscoring their significance in meeting the unique energy demands of each tissue. Notably, the brain exhibits the highest mtDNA copy numbers and an abundance of mitochondrial mRNAs of our earlier findings, potentially linked to the significant upregulation of mt-tRNAs in brain. This suggests a plausible association between mtDNA replication and the regulation of mtDNA gene expression.
CONCLUSIONS: Overall, our study unveils the tissue-specific expression of mitochondrial-encoded non-coding RNAs in buffalo. As we proceed, our further investigations into tissue-specific mitochondrial proteomics and microRNA studies aim to elucidate the intricate mechanisms within mitochondria, contributing to tissue-specific mitochondrial attributes. This research holds promise to elucidate the critical role of mitochondria in animal health and disease.