Abstract：
Since 2019, Lumpy skin disease (LSD) has suddenly spread in many Asian countries, including India. LSD primarily occurs in cattle. However, recent LSD outbreaks in India have also revealed significant morbidity and production losses in buffaloes. This has raised concerns about the role of buffaloes in the epidemiology and transmission of LSD and necessitates the inclusion of buffaloes in the mass vaccination program for the prevention and control of the disease in the country. However, there is no significant data on the immune response in buffaloes following vaccination with the LSD vaccine. In this study, we evaluated antibody- and cell-mediated immune responses following vaccination with a newly developed live-attenuated LSD vaccine (Lumpi-ProVacInd). The detectable amount of anti-LSDV antibodies was observed at 1-2 months following vaccination, with a peak antibody titer at 3 months. Upon stimulation of the peripheral blood mononuclear cells (PBMCs) with the UV-inactivated LSDV antigen, there was a significant increase in CD8 + T cell counts in vaccinated animals as compared to the unvaccinated animals. Besides, vaccinated animals also showed a significant increase in IFN-γ levels upon antigenic stimulation of their PBMCs with LSDV antigen. In conclusion, the buffaloes also mount a potent antibody- and cell-mediated immune response following vaccination with Lumpi-ProVacInd.
摘要：
自2019年以来，块状皮肤病（LSD）突然在许多亚洲国家蔓延，包括印度。LSD主要发生在牛身上。然而,最近在印度爆发的LSD也揭示了水牛的严重发病率和产量损失。这引起了人们对水牛在LSD的流行病学和传播中的作用的关注，因此有必要将水牛纳入大规模疫苗接种计划，以预防和控制该国的疾病。然而,没有关于接种LSD疫苗后水牛免疫反应的显著数据.在这项研究中,我们评估了新开发的LSD减毒活疫苗(Lumpi-ProVacInd)接种后抗体和细胞介导的免疫反应.在接种疫苗后1-2个月观察到可检测量的抗LSDV抗体。3个月时抗体滴度达到峰值。用紫外线灭活的LSDV抗原刺激外周血单核细胞（PBMC）后，与未接种疫苗的动物相比,接种疫苗的动物中CD8+T细胞计数显著增加.此外,接种疫苗的动物在用LSDV抗原刺激其PBMC后还显示IFN-γ水平的显著增加。总之,接种Lumpi-ProVacInd疫苗后，水牛也会产生有效的抗体和细胞介导的免疫反应。
