背景:在畜牧业中,异物综合症是一种毁灭性的疾病。喂养管理,缺乏食物歧视,吃切碎的食物会增加吞咽牛类尖锐异物的风险。除了蜂窝状的网状细胞形状,网状壁的收缩,妊娠子宫压力,和分娩努力,异物可以穿透网状壁,导致一系列问题,包括创伤性网膜炎,外伤性网状腹膜炎,和外伤性心包炎.本研究旨在评估心肌肌钙蛋白I快速检测盒和其他心脏生物标志物(包括血清心肌肌钙蛋白I(cTnI))的诊断意义。肌酸激酶-心肌带(CK-MB),乳酸脱氢酶(LDH),和天冬氨酸氨基转移酶(AST),牛和水牛的外伤性心包炎(TP)和/或外伤性网状腹膜炎(TRP)的确诊病例。
方法:总数量为30只动物(22头牛和8只水牛),表现为厌食症,颈静脉扩张,胸膜水肿,和疼痛的迹象(不愿移动,向后拱,和前肢外展)包括在本研究中。根据案例历史,临床体征,铁磁,心包穿刺术,放射和超声检查,TP在牛(n=10)和水牛(n=8)中得到证实,而TRP仅在牛(n=12)中得到证实。此外,使用20只临床健康动物(n=10头牛和10只水牛)作为对照组。收集血样用于测定Tn-I的血液水平,和CK-MB的活性,LDH,和AST。
结果:获得的结果表明,TP和TRP的患病牛血清cTnI显着增加(P=0.00),TP水牛血清cTnI无明显变化(P=0.111)。病牛和水牛均显示CK-MB的血清活性增加,AST,和LDH酶。另一方面,心肌肌钙蛋白I快速检测盒未能在患病动物中检测到cTnI。
结论:该研究的结论是,心肌肌钙蛋白I快速检测盒不具有诊断意义,并且不能在现场条件下用作诊断大型反刍动物TP和TRP的现场护理点。然而,血清肌钙蛋白I水平有助于诊断牛TP和TRP。尽管心脏生物标志物对TP和TRP有一定的诊断价值,传统的诊断方法(临床,射线照相和超声检查)对于全面评估牛的TP/TRP病例至关重要。
BACKGROUND: In the livestock industry, Foreign Body Syndrome is a devastating disease condition. Feeding management, lacking of food discrimination, and eating chopped food increase the risk of swallowing sharp foreign bodies in bovine species. In addition to the honeycomb cells shape of the reticulum, the contractions of the reticular wall, gravid uterine pressure, and parturition efforts, foreign bodies can penetrate the reticular wall, causing cascade of problems including traumatic reticulitis, traumatic reticuloperitonitis, and traumatic pericarditis. The present study was carried out to evaluate the diagnostic significance of cardiac troponin I rapid test cassette and other cardiac biomarkers including serum cardiac troponin I (cTn I), creatine kinase-myocardial band (CK-MB), lactate dehydrogenase (LDH), and aspartate aminotransferase enzyme (AST), in confirmed cases of traumatic pericarditis (TP) and/or traumatic reticuleoperitonitis (TRP) in cattle and
buffaloes.
METHODS: A total number of 30 animals (22 cattle and 8
buffaloes) with different signs such as anorexia, jugular distension, brisket edema, and signs of pain (reluctance to move, arching back, and abduction of the forelimbs) were included in the present study. Based on case history, clinical signs, ferroscopic, pericardiocentesis, radiographic and ultrasonographic examinations, TP were confirmed in cattle (n = 10) and buffaloes (n = 8) while TRP were confirmed only in cattle (n = 12). Additionally, 20 clinically healthy animals (n = 10 cattle and 10
buffaloes) were used as a control group. Blood samples were collected for determination of blood level of Tn-I, and activity of CK-MB, LDH, and AST.
RESULTS: The obtained results revealed a highly significant increase in serum cTn I in diseased cattle with TP and TRP (P = 0.00), while
buffaloes with TP showed no significant changes in serum cTn I (P = 0.111). Both diseased cattle and
buffaloes showed increased serum activities of CK-MB, AST, and LDH enzyme. On the other hand, cardiac troponin I rapid test cassette failed to detect cTn I in diseased animals.
CONCLUSIONS: The study concluded that the cardiac troponin I rapid test cassette did not have a diagnostic significance and could not be used as a point-of-care under field condition for diagnosis of TP and TRP in large ruminants. However, the serum troponin I level is helpful in diagnosis of TP and TRP in cattle. Although cardiac biomarkers have some diagnostic values in TP and TRP, the traditional diagnostic methods (clinical, radiography and ultrasonography examinations) are crucial for thorough evaluation of TP/TRP cases in bovine.