Abstract：
RNA isolation is an essential first step for many types of molecular analyses, including reverse transcription PCR (RT-PCR)/quantitative RT-PCR (qRT-PCR), Northern blotting, microarrays, and RNA-sequencing. While many RNA purification methods have been reported, it can be challenging to extract sufficient quantity, and suitable quality, of RNA from very small amounts of tissue and/or samples containing low numbers of cells. Here we outline a total RNA isolation method that reproducibly yields high-quality RNA from human stem cell-derived retinal organoids for downstream transcriptomic analysis.
摘要：
RNA分离是许多类型的分子分析必不可少的第一步，包括逆转录PCR（RT-PCR）/定量RT-PCR（qRT-PCR），北方印迹,微阵列,和RNA测序。虽然已经报道了许多RNA纯化方法，提取足够的量可能是具有挑战性的，和合适的质量，来自含有少量细胞的非常少量的组织和/或样品的RNA。在这里，我们概述了一种总RNA分离方法，该方法可重复地从人类干细胞衍生的视网膜类器官中产生高质量的RNA，用于下游转录组学分析。
