背景:牙髓干细胞(DPSC)具有自我更新和多向分化潜能。因此,DPSC具有广泛的临床应用。低水平激光治疗(LLLT)对细胞增殖具有积极的光生物刺激作用,血管生成,成骨分化,骨再生,和骨折愈合。然而,关于低能激光对DPSC增殖的影响的研究很少。
方法:从牙髓组织获得DPSC。通过体外培养和激光照射研究了LLLT对DPSCs增殖的影响及其相关机制。
结果:能量密度为3.5J/cm2和14J/cm2的LLLT促进了DPSC的增殖。差异蛋白表达研究表明,LLLT刺激DPSC增殖涉及PI3K-Akt和Rap1信号通路,以及凋亡相关途径。
结论:这项初步研究表明,低能激光对DPSC具有促增殖作用,并确定了可能的相关机制。本研究结果为DPSCs的临床应用提供了理论依据,为相关疾病的治疗提供了新的策略。
BACKGROUND: Dental pulp stem cells (DPSCs) have self-renewal and multidirectional differentiation potentials. As such, DPSCs have a wide range of clinical applications. Low-level laser therapy (LLLT) has positive photobiostimulatory effects on cell proliferation, angiogenesis, osteogenic differentiation, bone regeneration, and fracture healing. However, there have been few studies on the effect of low-energy lasers on DPSC proliferation.
METHODS: DPSCs were obtained from dental pulp tissue. The effects of LLLT on the proliferation of DPSCs and the associated mechanisms were investigated by in vitro culture and laser irradiation.
RESULTS: LLLT with energy densities of 3.5 J/cm2 and 14 J/cm2promoted the proliferation of DPSCs. Differential protein expression studies suggested the stimulation of DPSC proliferation by LLLT involved the PI3K-Akt and Rap1 signaling pathways, as well as the apoptosis-related pathway.
CONCLUSIONS: This preliminary study demonstrated that low-energy lasers have a pro-proliferative effect on DPSCs, and identified possible associated mechanisms. Our findings provide a theoretical basis for the clinical application of DPSCs and suggest novel strategies for the treatment of related diseases.