{Reference Type}: Journal Article
{Title}: RNA Isolation from Human Stem Cell-Derived Retinal Organoids.
{Author}: Keuthan CJ;Zack DJ;
{Journal}: Methods Mol Biol
{Volume}: 2822
{Issue}: 0
{Year}: 2024
暂无{DOI}: 10.1007/978-1-0716-3918-4_1
{Abstract}: RNA isolation is an essential first step for many types of molecular analyses, including reverse transcription PCR (RT-PCR)/quantitative RT-PCR (qRT-PCR), Northern blotting, microarrays, and RNA-sequencing. While many RNA purification methods have been reported, it can be challenging to extract sufficient quantity, and suitable quality, of RNA from very small amounts of tissue and/or samples containing low numbers of cells. Here we outline a total RNA isolation method that reproducibly yields high-quality RNA from human stem cell-derived retinal organoids for downstream transcriptomic analysis.