%0 Journal Article
%T RNA Isolation from Human Stem Cell-Derived Retinal Organoids.
%A Keuthan CJ
%A Zack DJ
%J Methods Mol Biol
%V 2822
%N 0
%D 2024
%M 38907907
暂无%R 10.1007/978-1-0716-3918-4_1
%X RNA isolation is an essential first step for many types of molecular analyses, including reverse transcription PCR (RT-PCR)/quantitative RT-PCR (qRT-PCR), Northern blotting, microarrays, and RNA-sequencing. While many RNA purification methods have been reported, it can be challenging to extract sufficient quantity, and suitable quality, of RNA from very small amounts of tissue and/or samples containing low numbers of cells. Here we outline a total RNA isolation method that reproducibly yields high-quality RNA from human stem cell-derived retinal organoids for downstream transcriptomic analysis.