anatolicumHyalommaanatolicum,安纳托利亚硬蜱是一种公认的媒介,涉及各种病原体向动物和人类的传播。本研究基于线粒体大亚基核糖体RNA(16SrRNA)基因序列,阐明了土豆菌的种群结构和单倍型网络。GenBank中存档的75个序列的种群结构和单倍型网络分析,包括本文生成的15个序列,产生24单倍型。单倍型1(Hap_1)是由来自印度的45个序列组成的主要单倍型,中国,巴基斯坦,土耳其,埃及,伊拉克,塔吉克斯坦。完整的单倍型网络表现出星状构象,突出了最近的人口扩张。整个数据集,以及对应于印度的序列,中国,巴基斯坦,显示出高单倍型(分别为0.638±0.065、0.671±0.103、0.753±0.099和0.854±0.061)和低核苷酸(分别为0.00407±0.00090、0.00525±0.00196、0.00680±0.00233和0.00453±0.00056)多样性,进一步强调了最近的人口扩张。包括田岛的D在内的中立性指数,傅和丽的D,完整数据集(分别为-2.661,-6.008和-5.649)以及来自印度的序列(分别为-2.223,-3.414和-3.567)的Fu和Li\的F为阴性,表明偏离中立和最近的人口膨胀。本研究提供了基于线粒体16SrRNA基因的种群结构和单倍型网络的新见解。不同的测试推断遗传分化较低,并表明这种经济上重要的蜱物种最近种群扩大。
Hyalomma anatolicum, an Anatolian hard tick is a well-recognized vector involved in the transmission of various pathogens to animals and humans. The present study elucidated the population structure and haplotype network of H. anatolicum based on the mitochondrial large subunit ribosomal RNA (16S rRNA) gene sequence. The population structure and haplotype network analysis of 75 sequences archived in the GenBank, including the 15 sequences generated herein, yielded 24 haplotypes. Haplotype 1 (Hap_1) was the predominant haplotype consisting of 45 sequences from India, China, Pakistan, Turkey, Egypt, Iraq, and Tajikistan. The complete haplotype network exhibited a stellate conformation, highlighting a recent population expansion. The overall dataset, together with the sequences corresponding to India, China, and Pakistan, showed a high haplotype (0.638 ± 0.065, 0.671 ± 0.103, 0.753 ± 0.099, and 0.854 ± 0.061, respectively) and low nucleotide (0.00407 ± 0.00090, 0.00525 ± 0.00196, 0.00680 ± 0.00233, and 0.00453 ± 0.00056, respectively) diversity, further emphasized a recent population expansion. The neutrality indices including Tajima\'s D, Fu and Li\'s D, and Fu and Li\'s F for the complete dataset (- 2.661, - 6.008, and - 5.649, respectively) as well as for the sequences from India (- 2.223, - 3.414, and - 3.567, respectively) were negative, suggesting deviation from neutrality and a recent population expansion. The present study provided novel insights into the population structure and haplotype networks of H. anatolicum based on the mitochondrial 16S rRNA gene, and the different tests inferred a low genetic differentiation and suggested a recent population expansion of this economically important tick species.