Abstract：
The abuse of methamphetamine is a significant threat to cardiovascular health and has detrimental effects on the myocardium. The present study aims to explore potential interventions that can mitigate myocardial pyroptosis in rats following methamphetamine withdrawal. A total of 104 male Wistar rats were randomly assigned to eight groups. The rats underwent a methamphetamine administration protocol, receiving intraperitoneal injections of 10 mg/kg during the 1st week, followed by a weekly dose escalation of 1 mg/kg from the second to the 6th week and two times per day. Concurrently, the rats engaged in 6 weeks of moderate-intensity treadmill aerobic training, lasting 60 min per day, 5 days a week. Simultaneously, the Nutrition bio-shield Superfood (NBS) supplement was administered at a dosage of 25 g/kg daily for 6 weeks. The study assessed the expression levels of Caspase-1, Interleukin-1beta (IL-1β), and Interleukin-18 (IL-18) genes in myocardial tissue. Data analysis utilized a one-way analysis of variance (p ≤ 0.05). The findings revealed that methamphetamine usage significantly elevated the expression of Caspase-1, IL-1β, and IL-18 genes (p ≤ 0.05). Conversely, methamphetamine withdrawal led to a notable reduction in the expression of these genes (p ≤ 0.05). Noteworthy reductions in Caspase-1, IL-1β, and IL-18 expression were observed following aerobic training, supplementation, and the combined approach (p ≤ 0.05). The chronic use of methamphetamine was associated with cardiac tissue damage. This study highlights the potential of aerobic training and NBS Superfood supplementation in mitigating the harmful effects of methamphetamine-induced myocardial pyroptosis. The observed reductions in gene expression levels indicate promising interventions to address the cardiovascular consequences of methamphetamine abuse. The findings of this study suggest that a combination of aerobic exercise and NBS Superfood supplementation can provide a promising approach to mitigate the deleterious effects of methamphetamine on the heart. These findings can be useful for healthcare professionals and policymakers to design effective interventions to prevent and manage the adverse effects of methamphetamine abuse.
摘要：
甲基苯丙胺的滥用是对心血管健康的重大威胁，并对心肌产生有害影响。本研究旨在探索可以减轻甲基苯丙胺戒断后大鼠心肌功能亢进的潜在干预措施。将104只雄性Wistar大鼠随机分为8组。大鼠接受了甲基苯丙胺给药方案，在第1周期间接受腹膜内注射10mg/kg，然后从第2周到第6周每周增加1mg/kg的剂量，每天两次。同时,大鼠进行6周中等强度的跑步机有氧训练,每天持续60分钟，一周五天。同时,营养生物防护超级食品（NBS）补充剂每天25g/kg，持续6周。该研究评估了Caspase-1，白细胞介素-1β（IL-1β）的表达水平，和心肌组织中的白细胞介素-18(IL-18)基因。数据分析采用单向方差分析(p≤0.05)。研究结果表明，甲基苯丙胺的使用显着升高了Caspase-1，IL-1β的表达，和IL-18基因（p≤0.05）。相反，甲基苯丙胺戒断导致这些基因的表达显着降低（p≤0.05）。值得注意的是Caspase-1，IL-1β，有氧训练后观察到IL-18表达，补充,和联合方法(p≤0.05)。慢性使用甲基苯丙胺与心脏组织损伤有关。这项研究强调了有氧训练和NBSSuperfood补充剂在减轻甲基苯丙胺诱导的心肌焦亡的有害影响方面的潜力。观察到的基因表达水平下降表明有希望的干预措施可以解决甲基苯丙胺滥用的心血管后果。这项研究的结果表明，有氧运动和NBSSuperfood补充剂的结合可以提供一种有希望的方法来减轻甲基苯丙胺对心脏的有害影响。这些发现有助于医疗保健专业人员和政策制定者设计有效的干预措施，以预防和管理甲基苯丙胺滥用的不利影响。
