背景:山楂果实是一种有趣的药用植物,具有多种生物学特征,特别是与抗炎有关的,抗氧化和免疫调节作用,促进整体健康。在这项研究中,旨在阐明山楂醋对机体免疫及整体健康的影响。我们还重点研究了三种不同的生产工艺来提高山楂醋的抗氧化活性(2)方法:在研究中,除了传统的山楂醋(N)生产外,将热巴氏灭菌(P)和超声(U)技术应用于醋。雌性成年Wistar白化病大鼠56只,随机分为7组,N0.5(普通醋;0.5mL/kgbw),N1(普通醋;1mL/kgbw),P0.5(巴氏杀菌醋;0.5mL/kgbw),P1(巴氏杀菌醋;1mL/kgbw),U0.5(超声处理的醋;0.5mL/kgbw),和U1(超声处理的醋;1mL/kgbw)。每天通过口服管饲法施用醋。平均体重增加,身体质量指数,并测量血液血液学参数,并计算中性粒细胞淋巴细胞比率。血浆IL-1β和TNF-α值,MDA,肠组织IL-1β和TNF-α值,决心。此外,应用链霉亲和素-生物素-过氧化物酶复合物法测定十二指肠中TNF-α和IL-1β的表达。(3)结果:与对照组相比,所有醋组的平均增重均呈下降趋势。此外,所有醋组中的NL比率都有所增加,虽然不重要。各食醋组之间无统计学差异,尽管在血浆IL-1β中观察到下降。此外,高剂量醋组(N1,P1和U1)的血浆TNF-α值略有增加,虽然不重要。此外,N0.5,N1和P0.5组肠组织IL-1β值趋于升高,而P1,U0.5和U1组IL-1β值趋于降低。另一方面,与对照组相比,所有组肠组织的TNF-α值均略有增加,尽管这些并不重要。此外,U0.5组和U1组均有TNF-α和IL-1β的表达。(4)结论:结果表明,高剂量或超声应用山楂醋对肠道健康有积极影响。提高免疫力和整体健康。
BACKGROUND: The hawthorn fruit is an interesting medicinal plant that has several biological features, especially related to anti-inflammatory, antioxidant and immune-modulating actions, and boosting general health. In this study, we aimed to clarify the immunological effects of hawthorn vinegar on immunity and general health. We also focused on three different production processes to improve the antioxidant activity of hawthorn vinegar (2) Methods: In the study, besides the traditional production of hawthorn vinegar (N), thermal pasteurization (P) and ultrasound (U) techniques were applied to vinegars. A total of 56 female adult Wistar albino rats were randomly allocated into seven groups; Control, N0.5 (regular vinegar; 0.5 mL/kgbw), N1 (regular vinegar; 1 mL/kgbw), P0.5 (pasteurized vinegar; 0.5 mL/kgbw), P1 (pasteurized vinegar; 1 mL/kgbw), U0.5 (ultrasound treated vinegar; 0.5 mL/kgbw), and U1 (ultrasound treated vinegar; 1 mL/kgbw). Vinegars were administered by oral gavage daily. The average weight gains, body mass index, and blood hematological parameters were measured, and the Neutrophil Lymphocyte ratio was calculated. The plasma IL-1β and TNF-α values, and MDA, IL-1β and TNF-α values of intestinal tissue, were determined. Also, the streptavidin-biotin-peroxidase complex method was applied to determine the expressions of TNF-α and IL-1β in duodenum. (3) Results: There was a decreasing tendency in the average weight gains in all vinegar groups compared to the control group. In addition, there was an increase in NL ratio in all vinegar groups, although not significant. There were no statistical differences among all vinegar groups, although decreases were observed in plasma IL-1β. Also, the plasma TNF-α values showed slight increases in high-dose-of-vinegar groups (N1, P1 and U1), although not significant. In addition, the intestinal tissue IL-1β value tended to increase in groups N0.5, N1 and P0.5, while it tended to decrease in P1, U0.5 and U1. On the other hand, there were slight increases in the TNF-α values of intestinal tissue in all groups compared to control, although these were not significant. Furthermore, the intensive expressions of TNF-α and IL-1β were determined in groups U0.5 and U1. (4) Conclusions: The results suggest that either high doses or ultrasound applications of hawthorn vinegar have positive effects on intestinal health, boosting immunity and general health.